ArticleJournal of synchrotron radiation2013
Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam.
Article in Journal of synchrotron radiation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
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- Time-resolved small-angle X-ray scattering system development for the biological macromolecules at SACLA: A pilot study.Biophysics and physicobiology · 2025Article
- Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death.Communications biology · 2024Article
- Serial X-ray liquidography: multi-dimensional assay framework for exploring biomolecular structural dynamics with microgram quantities.Nature communications · 2024Article
- Article
- Article
- Sample-minimizing co-flow cell for time-resolved pump-probe X-ray solution scattering.Journal of synchrotron radiation · 2023Article
- A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory.Journal of synchrotron radiation · 2022Article
- Wall-free droplet microfluidics for probing biological processes by high-brilliance X-ray scattering techniques.Frontiers in molecular biosciences · 2022Review
- A multi-step nucleation process determines the kinetics of prion-like domain phase separation.Nature communications · 2021Article
- Shear-free mixing to achieve accurate temporospatial nanoscale kinetics through scanning-SAXS: ion-induced phase transition of dispersed cellulose nanocrystals.Lab on a chip · 2021Article
- Article
- A novel experimental approach for nanostructure analysis: simultaneous small-angle X-ray and neutron scattering.Journal of applied crystallography · 2020Article
- BEES: Bayesian Ensemble Estimation from SAS.Biophysical journal · 2019Article
- The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation.Journal of synchrotron radiation · 2019Article
- Predicting data quality in biological X-ray solution scattering.Acta crystallographica. Section D, Structural biology · 2018Review
- Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions.Journal of synchrotron radiation · 2018Article
- A method for efficient Bayesian optimization of self-assembly systems from scattering data.BMC systems biology · 2018Article
- Microfluidic devices for small-angle neutron scattering.Journal of applied crystallography · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Small-angle X-ray scattering (SAXS) is a well established technique to probe the nanoscale structure and interactions in soft matter. It allows one to study the structure of native particles in near physiological environments and to analyze structural changes in response to variations in external conditions. The combination of microfluidics and SAXS provides a powerful tool to investigate dynamic processes on a molecular level with sub-millisecond time resolution. Reaction kinetics in the sub-millisecond time range has been achieved using continuous-flow mixers manufactured using micromachining techniques. The time resolution of these devices has previously been limited, in part, by the X-ray beam sizes delivered by typical SAXS beamlines. These limitations can be overcome using optics to focus X-rays to the micrometer size range providing that beam divergence and photon flux suitable for performing SAXS experiments can be maintained. Such micro-SAXS in combination with microfluidic devices would be an attractive probe for time-resolved studies. Here, the development of a high-duty-cycle scanning microsecond-time-resolution SAXS capability, built around the Kirkpatrick-Baez mirror-based microbeam system at the Biophysics Collaborative Access Team (BioCAT) beamline 18ID at the Advanced Photon Source, Argonne National Laboratory, is reported. A detailed description of the microbeam small-angle-scattering instrument, the turbulent flow mixer, as well as the data acquisition and control and analysis software is provided. Results are presented where this apparatus was used to study the folding of cytochrome c. Future prospects for this technique are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.