Evidence map›Paper›PMID 24120826›Full record

ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain.

Lanlan Wei, Ming Chu, Qingmeng Zhang, Yan Wang, Qinglong Shang, Yunyan Zhang, Guangmei Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Lanlan WeiDepartment of Microbiology, Harbin Medical University, Harbin, China; Immunity and Infection, Pathogenic Biology Key Laboratory of Heilongjiang Province, Harbin, China.
Ming ChuThe First Affiliated Hospital of Harbin Medical University, Harbin, China.
Qingmeng ZhangDepartment of Microbiology, Harbin Medical University, Harbin, China; Immunity and Infection, Pathogenic Biology Key Laboratory of Heilongjiang Province, Harbin, China; The First Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Gynecology, The Third Affiliated Hospital, Harbin Medical University, Harbin, China.
Yan WangDepartment of Microbiology, Harbin Medical University, Harbin, China; Immunity and Infection, Pathogenic Biology Key Laboratory of Heilongjiang Province, Harbin, China.
Qinglong ShangDepartment of Microbiology, Harbin Medical University, Harbin, China; Immunity and Infection, Pathogenic Biology Key Laboratory of Heilongjiang Province, Harbin, China.
Yunyan ZhangDepartment of Gynecology, The Third Affiliated Hospital, Harbin Medical University, Harbin, China. Electronic address: zhangyunyan1972@yahoo.com.cn.
Guangmei ZhangThe First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: guangmeizhang@126.com.
Harbin Medical University · CNEducation Department of Heilongjiang Province · CNFirst Affiliated Hospital of Harbin Medical University · CNThird Affiliated Hospital of Harbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12).

methodsThe variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4(+) and CD8(+) in spleen cells was detected with flow cytometry.

resultsThe titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16 VLP epitopes. Splenic CD4(+) and CD8(+) T cell subsets increased after vaccination in every experimental group, and CD8(+) increased obviously in L1P group. The ratio of CD4(+)/CD8(+) decreased in L1P group and increased in the other two groups, compared to control group. Vaccination induced specific secretion of IFN-γ in the serum of vaccinated group (p<0.05), especially in the L1P group.

conclusionsVLP of HPV16 variant strain isolated in East China could induce humoral immunity and cellular immunity in mice, and IL-12 recombinant plasmid can enhance cellular immunity.

Indexed as

Adjuvants, ImmunologicAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayFemaleFlow CytometryHuman papillomavirus 16HumansImmunity, CellularImmunoglobulin GInterleukin-12MiceMice, Inbred BALB CMolecular Sequence DataPapillomavirus InfectionsVaccines, Virus-Like ParticleAdjuvants, ImmunologicAntibodies, ViralImmunoglobulin GInterleukin-12Vaccines, Virus-Like ParticleCellular immunityCervical cancerHuman papillomavirus type 16IL-12Virus-like particle

Identifiers

PMID24120826
PMCPMC9425263
OpenAlexW2141305090

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.