ArticleGastroenterology2014
Proper development of the outer longitudinal smooth muscle of the mouse pylorus requires Nkx2-5 and Gata3.
Article in Gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Genome-wide meta-analysis identifies BARX1 and EML4-MTA3 as new loci associated with infantile hypertrophic pyloric stenosis.Human molecular genetics · 2019Pooled it
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- Genome-wide association study of mature cow size traits in American Angus cattle.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Article
- A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease.Human molecular genetics · 2019Article
- Co-occurrence of infantile hypertrophic pyloric stenosis and congenital heart defects: a nationwide cohort study.Pediatric research · 2019Article
- Are Gastric and Esophageal Metaplasia Relatives? The Case for Barrett's Stemming from SPEM.Digestive diseases and sciences · 2018Review
- Translating Developmental Principles to Generate Human Gastric Organoids.Cellular and molecular gastroenterology and hepatology · 2018Review
- Chick midgut morphogenesis.The International journal of developmental biology · 2018Review
- Stomach Organ and Cell Lineage Differentiation: from Embryogenesis to Adult Homeostasis.Cellular and molecular gastroenterology and hepatology · 2016Article
- Stomach development, stem cells and disease.Development (Cambridge, England) · 2016Review
- Mesenchymal-epithelial interactions during digestive tract development and epithelial stem cell regeneration.Cellular and molecular life sciences : CMLS · 2015Review
- Association between NKX2-5 rs29784 and infantile hypertrophic pyloric stenosis in Chinese Han population.International journal of clinical and experimental medicine · 2015Article
- Roles for Nkx2-5 and Gata3 in the ontogeny of the murine smooth muscle gastric ligaments.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3.BMC biology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
BACKGROUND &
aimsInfantile hypertrophic pyloric stenosis is a common birth anomaly characterized by obstruction of the pyloric lumen. A genome-wide association study implicated NKX2-5, which encodes a transcription factor that is expressed in embryonic heart and pylorus, in the pathogenesis of infantile hypertrophic pyloric stenosis. However, the function of the NKX2-5 in pyloric smooth muscle development has not been examined directly. We investigated the pattern of Nkx2-5 during the course of murine pyloric sphincter development and examined coexpression of Nkx2-5 with Gata3 and Sox9-other transcription factors with pyloric-specific mesenchymal expression. We also assessed pyloric sphincter development in mice with disruption of Nkx2-5 or Gata3.
methodsWe used immunofluorescence analysis to compare levels of NKX2-5, GATA3, and SOX9 in different regions of smooth muscle cells. Pyloric development was assessed in mice with conditional or germline deletion of Nkx2-5 or Gata3, respectively.
resultsGata3, Nkx2-5, and Sox9 are coexpressed in differentiating smooth muscle cells of a distinct fascicle of the pyloric outer longitudinal muscle. Expansion of this fascicle coincides with development of the pyloric sphincter. Disruption of Nkx2-5 or Gata3 causes severe hypoplasia of this fascicle and alters pyloric muscle shape. Although expression of Sox9 requires Nkx2-5 and Gata3, there is no apparent hierarchical relationship between Nkx2-5 and Gata3 during pyloric outer longitudinal muscle development.
conclusionsNkx2-5 and Gata3 are independently required for the development of a pyloric outer longitudinal muscle fascicle, which is required for pyloric sphincter morphogenesis in mice. These data indicate that regulatory changes that alter Nkx2-5 or Gata3 expression could contribute to pathogenesis of infantile hypertrophic pyloric stenosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.