ArticleJournal of nanobiotechnology2013
Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2.
Article in Journal of nanobiotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
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- Regulation of nanomaterials in peripheral nerve regeneration from a microenvironmental perspective: biological effects and mechanisms.Materials today. Bio · 2025Review
- Nanotopographical Features of Polymeric Nanocomposite Scaffolds for Tissue Engineering and Regenerative Medicine: A Review.Biomimetics (Basel, Switzerland) · 2025Review
- Mechanical and Biological Properties of Titanium and Its Alloys for Oral Implant with Preparation Techniques: A Review.Materials (Basel, Switzerland) · 2023Review
- GPR55 Receptor Activation by theInternational journal of molecular sciences · 2021Article
- PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.Cells · 2020Article
- Cluster-assembled zirconia substrates promote long-term differentiation and functioning of human islets of Langerhans.Scientific reports · 2018Article
- Biocompatible Gold Nanoparticles Ameliorate Retinoic Acid-Induced Cell Death and Induce Differentiation in F9 Teratocarcinoma Stem Cells.Nanomaterials (Basel, Switzerland) · 2018Article
- Intermediate and low abundant protein analysis of vitamin D deficient obese and non-obese subjects by MALDI-profiling.Scientific reports · 2017Article
- Proteomic profile of maternal-aged blastocoel fluid suggests a novel role for ubiquitin system in blastocyst quality.Journal of assisted reproduction and genetics · 2017Article
- Proteomic Dissection of Nanotopography-Sensitive Mechanotransductive Signaling Hubs that Foster Neuronal Differentiation in PC12 Cells.Frontiers in cellular neuroscience · 2017Article
- Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin.EuPA open proteomics · 2016Article
- Nanochips of Tantalum Oxide Nanodots as artificial-microenvironments for monitoring Ovarian cancer progressiveness.Scientific reports · 2016Article
- Conversion of nanoscale topographical information of cluster-assembled zirconia surfaces into mechanotransductive events promotes neuronal differentiation.Journal of nanobiotechnology · 2016Article
- Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome.EuPA open proteomics · 2016Article
- Scale Invariant Disordered Nanotopography Promotes Hippocampal Neuron Development and Maturation with Involvement of Mechanotransductive Pathways.Frontiers in cellular neuroscience · 2016Article
- Spatial Control of Cell-Nanosurface Interactions by Tantalum Oxide Nanodots for Improved Implant Geometry.PloS one · 2016Article
- Topological control of nitric oxide secretion by tantalum oxide nanodot arrays.Journal of nanobiotechnology · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSubstrate nanoscale topography influences cell proliferation and differentiation through mechanisms that are at present poorly understood. In particular the molecular mechanism through which cells 'sense' and adapt to the substrate and activate specific intracellular signals, influencing cells survival and behavior, remains to be clarified.
resultsTo characterize these processes at the molecular level we studied the differentiation of PC12 cells on nanostructured TiO2 films obtained by supersonic cluster beam deposition.Our findings indicate that, in PC12 cells grown without Nerve Growth Factor (NGF), the roughness of nanostructured TiO2 triggers neuritogenesis by activating the expression of nitric oxide synthase (NOS) and the phospho-extracellular signal-regulated kinase 1/2 (pERK1/2) signaling. Differentiation is associated with an increase in protein nitration as observed in PC12 cells grown on flat surfaces in the presence of NGF. We demonstrate that cell differentiation and protein nitration induced by topography are not specific for PC12 cells but can be regarded as generalized effects produced by the substrate on different neuronal-like cell types, as shown by growing the human neuroblastoma SH-SY5Y cell line on nanostructured TiO2.
conclusionOur data provide the evidence that the nitric oxide (NO) signal cascade is involved in the differentiation process induced by nanotopography, adding new information on the mechanism and proteins involved in the neuritogenesis triggered by the surface properties.
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