ArticleBMC plant biology2013
The identification of novel loci required for appropriate nodule development in Medicago truncatula.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 56 citations in OpenAlex.
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- Multiple sensors provide spatiotemporal oxygen regulation of gene expression in a Rhizobium-legume symbiosis.PLoS genetics · 2021Article
- Amino Acid Polymorphisms in the VHIID Conserved Motif of Nodulation Signaling Pathways 2 Distinctly Modulate Symbiotic Signaling and Nodule Morphogenesis inFrontiers in plant science · 2021Article
- The Medicago truncatula Vacuolar iron Transporter-Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process.The New phytologist · 2020Article
- A Select and Resequence Approach Reveals Strain-Specific Effects ofPlant physiology · 2020Article
- Allelic Variants for Candidate Nitrogen Fixation Genes Revealed by Sequencing in Red Clover (International journal of molecular sciences · 2019Article
- Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis.Plant biotechnology journal · 2019Article
- Characterization of Novel Plant Symbiosis Mutants Using a New Multiple Gene-Expression ReporterFrontiers in plant science · 2018Article
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- Validating Genome-Wide Association Candidates Controlling Quantitative Variation in Nodulation.Plant physiology · 2017Article
- A Proteomic View on the Role of Legume Symbiotic Interactions.Frontiers in plant science · 2017Review
- Article
- A Snapshot of Functional Genetic Studies in Medicago truncatula.Frontiers in plant science · 2016Review
- Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Multiple steps control immunity during the intracellular accommodation of rhizobia.Journal of experimental botany · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe formation of functional symbiotic nodules is the result of a coordinated developmental program between legumes and rhizobial bacteria. Genetic analyses in legumes have been used to dissect the signaling processes required for establishing the legume-rhizobial endosymbiotic association. Compared to the early events of the symbiotic interaction, less attention has been paid to plant loci required for rhizobial colonization and the functioning of the nodule. Here we describe the identification and characterization of a number of new genetic loci in Medicago truncatula that are required for the development of effective nitrogen fixing nodules.
resultsApproximately 38,000 EMS and fast neutron mutagenized Medicago truncatula seedlings were screened for defects in symbiotic nitrogen fixation. Mutant plants impaired in nodule development and efficient nitrogen fixation were selected for further genetic and phenotypic analysis. Nine mutants completely lacking in nodule formation (Nod-) represented six complementation groups of which two novel loci have been identified. Eight mutants with ineffective nodules (Fix-) represented seven complementation groups, out of which five were new monogenic loci. The Fix- M. truncatula mutants showed symptoms of nitrogen deficiency and developed small white nodules. Microscopic analysis of Fix- nodules revealed that the mutants have defects in the release of rhizobia from infection threads, differentiation of rhizobia and maintenance of persistence of bacteria in nodule cells. Additionally, we monitored the transcriptional activity of symbiosis specific genes to define what transcriptional stage of the symbiotic process is blocked in each of the Fix- mutants. Based on the phenotypic and gene expression analysis a functional hierarchy of the FIX genes is proposed.
conclusionsThe new symbiotic loci of M. truncatula isolated in this study provide the foundation for further characterization of the mechanisms underpinning nodulation, in particular the later stages associated with bacterial release and nodule function.
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