ArticleBMC complementary and alternative medicine2013
Neurite outgrowth stimulatory effects of culinary-medicinal mushrooms and their toxicity assessment using differentiating Neuro-2a and embryonic fibroblast BALB/3T3.
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- Neuritogenic effects of Ulva lactuca aqueous extract on Neuro-2a cells.BMC complementary medicine and therapies · 2026Article
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- Potential Hemostatic and Wound Healing Effects of Thermoresponsive Wound Dressing Gel Loaded withGels (Basel, Switzerland) · 2023Article
- Biological Synthesis of Low Cytotoxicity Silver Nanoparticles (AgNPs) by the FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Regulator of G-Protein Signaling-4 Attenuates Cardiac Adverse Remodeling and Neuronal Norepinephrine Release-Promoting Free Fatty Acid Receptor FFAR3 Signaling.International journal of molecular sciences · 2022Article
- Intrinsically disordered intracellular domains control key features of the mechanically-gated ion channel PIEZO2.Nature communications · 2022Article
- The complete mitochondrial genome of the edible mushroomMitochondrial DNA. Part B, Resources · 2022Article
- In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications.Scientific reports · 2021Article
- SIRT1 Activation by Polydatin Alleviates Oxidative Damage and Elevates Mitochondrial Biogenesis in Experimental Diabetic Neuropathy.Cellular and molecular neurobiology · 2021Article
- Exploration of nutritional, antioxidative, antibacterial and anticancer status ofJournal of food science and technology · 2021Article
- Reductive stress promotes protein aggregation and impairs neurogenesis.Redox biology · 2020Article
- Hericium erinaceus potentially rescues behavioural motor deficits through ERK-CREB-PSD95 neuroprotective mechanisms in rat model of 3-acetylpyridine-induced cerebellar ataxia.Scientific reports · 2020Article
- Potential Antidepressant Effects of Scutellaria baicalensis, Hericium erinaceus and Rhodiola rosea.Antioxidants (Basel, Switzerland) · 2020Review
- Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study.BMC complementary and alternative medicine · 2019Article
- Lion's Mane Mushroom,Antioxidants (Basel, Switzerland) · 2019Article
- Human Embryonic Stem Cell-Derived Neural Lineages asBioMed research international · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMushrooms are not only regarded as gourmet cuisine but also as therapeutic agent to promote cognition health. However, little toxicological information is available regarding their safety. Therefore, the aim of this study was to screen selected ethno-pharmacologically important mushrooms for stimulatory effects on neurite outgrowth and to test for any cytotoxicity.
methodsThe stimulatory effect of mushrooms on neurite outgrowth was assessed in differentiating mouse neuroblastoma (N2a) cells. Neurite length was measured using Image-Pro Insight processor system. Neuritogenesis activity was further validated by fluorescence immunocytochemical staining of neurofilaments. In vitro cytotoxicity was investigated by using mouse embryonic fibroblast (BALB/3T3) and N2a cells for any embryo- and neuro-toxic effects; respectively.
resultsAqueous extracts of Ganoderma lucidum, Lignosus rhinocerotis, Pleurotus giganteus and Grifola frondosa; as well as an ethanol extract of Cordyceps militaris significantly (p < 0.05) promoted the neurite outgrowth in N2a cells by 38.4 ± 4.2%, 38.1 ± 2.6%, 33.4 ± 4.6%, 33.7 ± 1.5%, and 35.8 ± 3.4%; respectively. The IC50 values obtained from tetrazolium (MTT), neutral red uptake (NRU) and lactate dehydrogenase (LDH) release assays showed no toxic effects following 24 h exposure of N2a and 3T3 cells to mushroom extracts.
conclusionOur results indicate that G. lucidum, L. rhinocerotis, P. giganteus, G. frondosa and C. militaris may be developed as safe and healthy dietary supplements for brain and cognitive health.
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