ArticleEnvironmental microbiology2014
Architecture and host interface of environmental chlamydiae revealed by electron cryotomography.
Article in Environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Stable association of a chlamydial symbiont with the freshwater predator hydra suggests broad host potential.The ISME journal · 2026Article
- Obligate intracellular bacteria and host cell death pathways-the matter of life and death.Cell and tissue research · 2025Review
- Identification and characterization of a ubiquitin E3 RING ligase of the Chlamydia-like bacterium Simkania negevensis.PLoS pathogens · 2025Article
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- All-Round Manipulation of the Actin Cytoskeleton by HIV.Viruses · 2018Review
- Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells.Pathogens and disease · 2017Article
- Bacterial cell biology outside the streetlight.Environmental microbiology · 2016Review
- Compartmentalization in PVC super-phylum: evolution and impact.Biology direct · 2016Article
- A new view into prokaryotic cell biology from electron cryotomography.Nature reviews. Microbiology · 2016Review
- A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.Biophysical journal · 2016Article
- Developmental Cycle and Genome Analysis of "Rubidus massiliensis," a New Vermamoeba vermiformis Pathogen.Frontiers in cellular and infection microbiology · 2016Article
- Chlamydia trachomatis Relies on Autonomous Phospholipid Synthesis for Membrane Biogenesis.The Journal of biological chemistry · 2015Article
- Chlamydiae interaction with the endoplasmic reticulum: contact, function and consequences.Cellular microbiology · 2015Review
- STIM1 Is a Novel Component of ER-Chlamydia trachomatis Inclusion Membrane Contact Sites.PloS one · 2015Article
- Chlamydia infection depends on a functional MDM2-p53 axis.Nature communications · 2014Article
- Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography.Cellular microbiology · 2014Article
- Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities.FEMS microbiology reviews · 2014Review
- Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography.Nature methods · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Chlamydiae comprise important pathogenic and symbiotic bacteria that alternate between morphologically and physiologically different life stages during their developmental cycle. Using electron cryotomography, we characterize the ultrastructure of the developmental stages of three environmental chlamydiae: Parachlamydia acanthamoebae, Protochlamydia amoebophila and Simkania negevensis. We show that chemical fixation and dehydration alter the cell shape of Parachlamydia and that the crescent body is not a developmental stage, but an artefact of conventional electron microscopy. We further reveal type III secretion systems of environmental chlamydiae at macromolecular resolution and find support for a chlamydial needle-tip protein. Imaging bacteria inside their host cells by cryotomography for the first time, we observe marked differences in inclusion morphology and development as well as host organelle recruitment between the three chlamydial organisms, with Simkania inclusions being tightly enveloped by the host endoplasmic reticulum. The study demonstrates the power of electron cryotomography to reveal structural details of bacteria-host interactions that are not accessible using traditional methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.