ArticleMolecular plant pathology2014
Overexpression of GmCaM4 in soybean enhances resistance to pathogens and tolerance to salt stress.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 105 citations in OpenAlex.
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- Calcium signaling in soybean-virus interactions: emerging roles of calcium sensors in antiviral and environmental stress responses.Frontiers in plant science · 2026Review
- Genome-wide study of salt tolerance in USDA tomato germplasm at seedling stage.Scientific reports · 2025Article
- Zea Maize Calmodulin (Plants (Basel, Switzerland) · 2025Article
- Screening of salt-tolerant soybean germplasm and study of salt-tolerance mechanism.Plant cell reports · 2025Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2025Article
- Comprehensive in-silico characterization and expression pattern of calmodulin genes under various abiotic and biotic stresses in Indian mustard (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- QTL Detection of Salt Tolerance at Soybean Seedling Stage Based on Genome-Wide Association Analysis and Linkage Analysis.Plants (Basel, Switzerland) · 2024Article
- Genome-wide identification and expression analysis of calmodulin and calmodulin-like genes in passion fruit (Passiflora edulis) and their involvement in flower and fruit development.BMC plant biology · 2024Article
- Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation.Plants (Basel, Switzerland) · 2024Article
- Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress.Plants (Basel, Switzerland) · 2023Review
- From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches.Metabolites · 2023Review
- Article
- Twenty years of mining salt tolerance genes in soybean.Molecular breeding : new strategies in plant improvement · 2023Article
- The calcium connection: exploring the intricacies of calcium signaling in plant-microbe interactions.Frontiers in plant science · 2023Review
- Comparative transcriptome profiling reveals differential defense responses amongFrontiers in plant science · 2023Article
- Article
- Genome-wide identification and expression analysis of calmodulin and calmodulin-like genes in wheat (Plant signaling & behavior · 2022Article
- Genome-Wide Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2022Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant diseases inflict heavy losses on soybean yield, necessitating an understanding of the molecular mechanisms underlying biotic/abiotic stress responses. Ca(2) (+) is an important universal messenger, and protein sensors, prominently calmodulins (CaMs), recognize cellular changes in Ca(2) (+) in response to diverse signals. Because the development of stable transgenic soybeans is laborious and time consuming, we used the Bean pod mottle virus (BPMV)-based vector for rapid and efficient protein expression and gene silencing. The present study focuses on the functional roles of the gene encoding the soybean CaM isoform GmCaM4. Overexpression of GmCaM4 in soybean resulted in enhanced resistance to three plant pathogens and increased tolerance to high salt conditions. To gain an understanding of the underlying mechanisms, we examined the potential defence pathways involved. Our studies revealed activation/increased expression levels of pathogenesis-related (PR) genes in GmCaM4-overexpressing plants and the accumulation of jasmonic acid (JA). Silencing of GmCaM4, however, markedly repressed the expression of PR genes. We confirmed the in vivo interaction between GmCaM4 and the CaM binding transcription factor Myb2, which regulates the expression of salt-responsive genes, using the yeast two-hybrid (Y2H) system and bimolecular fluorescence complementation assays. GmCaM4 and Glycine max CaM binding receptor-like kinase (GmCBRLK) did not interact in the Y2H assays, but the interaction between GmCaM2 and GmCBRLK was confirmed. Thus, a GmCaM2-GmCBRLK-mediated salt tolerance mechanism, similar to that reported in Glycine soja, may also be functional in soybean. Confocal microscopy showed subcellular localization of the green fluorescent protein (GFP)-GmCaM4 fusion protein in the nucleus and cytoplasm.
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