ArticleTissue engineering. Part C, Methods2014
Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Assessing bone regeneration potential of 3D scaffold-free cell pellets from periodontal ligament and bone marrow stem cells.BMC biotechnology · 2025Article
- Fabrication of 3D Collagen-Based Decellularized Biological Scaffolds Using Human Wharton's Jelly-Derived Mesenchymal Stem Cells With Differentiation Potential Toward Chondrocytes.Stem cells international · 2025Article
- The application of ECM-derived biomaterials in cartilage tissue engineering.Mechanobiology in medicine · 2023Review
- Decellularized Extracellular Matrix: The Role of This Complex Biomaterial in Regeneration.ACS omega · 2023Review
- Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes' Phenotype in a ScaffoldSovremennye tekhnologii v meditsine · 2023Article
- Articular Cartilage Regeneration in Veterinary Medicine.Advances in experimental medicine and biology · 2022Article
- Zone-Dependent Architecture and Biochemical Composition of Decellularized Porcine Nasal Cartilage Modulate the Activity of Adipose Tissue-Derived Stem Cells in Cartilage Regeneration.International journal of molecular sciences · 2021Article
- Chondrogenesis of cocultures of mesenchymal stem cells and articular chondrocytes in poly(l-lysine)-loaded hydrogels.Journal of controlled release : official journal of the Controlled Release Society · 2020Article
- Review
- Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine.Frontiers in bioengineering and biotechnology · 2020Review
- Biomimetic poly(glycerol sebacate)/polycaprolactone blend scaffolds for cartilage tissue engineering.Journal of materials science. Materials in medicine · 2019Article
- Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources.Acta biomaterialia · 2018Review
- Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro.International journal of molecular medicine · 2018Article
- Combating Osteoarthritis through Stem Cell Therapies by Rejuvenating Cartilage: A Review.Stem cells international · 2018Review
- Review
- Cell type-specific extracellular matrix guided the differentiation of human mesenchymal stem cells in 3D polymeric scaffolds.Journal of materials science. Materials in medicine · 2017Article
- The Challenge in Using Mesenchymal Stromal Cells for Recellularization of Decellularized Cartilage.Stem cell reviews and reports · 2017Review
- Approaching the compressive modulus of articular cartilage with a decellularized cartilage-based hydrogel.Acta biomaterialia · 2016Article
- Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage.Annals of biomedical engineering · 2016Article
- Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes.Tissue engineering. Part A · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds. Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days. These constructs were then devitalized to isolate the chondrogenic effects of the ECM alone. Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds. Following devitalization, the PCL/ECM scaffolds were then cultured with MSCs in serum-free conditions with or without TGF-β3 treatment for 21 days. TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings. Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone. These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls. This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.