ArticlePloS one2013
In vitro grown pollen tubes of Nicotiana alata actively synthesise a fucosylated xyloglucan.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction.Plant reproduction · 2026Review
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan.The New phytologist · 2023Article
- Identification of a xyloglucan beta-xylopyranosyltransferase fromPlant direct · 2023Article
- Dynamics of cell wall polysaccharides during the elongation growth of rye primary roots.Planta · 2022Article
- Article
- Ancient origin of fucosylated xyloglucan in charophycean green algae.Communications biology · 2021Article
- Biochemical and Functional Characterization of GALT8, anFrontiers in plant science · 2021Article
- Plant ProteinFrontiers in plant science · 2021Review
- Xyloglucan exoglycosidases in the monocot model Brachypodium distachyon and the conservation of xyloglucan disassembly in angiosperms.Plant molecular biology · 2019Article
- Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling.Frontiers in plant science · 2019Review
- Monitoring Polysaccharide Dynamics in the Plant Cell Wall.Plant physiology · 2018Review
- Identification of an arabinopyranosyltransferase fromPlant direct · 2018Article
- Article
- A Glycosyltransferase from Nicotiana alata Pollen Mediates Synthesis of a Linear (1,5)-α-L-Arabinan When Expressed in Arabidopsis.Plant physiology · 2016Article
- The presence of fucogalactoxyloglucan and its synthesis in rice indicates conserved functional importance in plants.Plant physiology · 2015Article
- Article
- Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan.Annals of botany · 2015Article
- In silico prediction of proteins related to xyloglucan fucosyltransferases in Solanaceae genomes.Plant signaling & behavior · 2015Article
- Structural Diversity and Function of Xyloglucan Sidechain Substituents.Plants (Basel, Switzerland) · 2014Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotiana alata pollen tubes are a widely used model for studies of polarized tip growth and cell wall synthesis in plants. To better understand these processes, RNA-Seq and de novo assembly methods were used to produce a transcriptome of N. alata pollen grains. Notable in the reconstructed transcriptome were sequences encoding proteins that are involved in the synthesis and remodelling of xyloglucan, a cell wall polysaccharide previously not thought to be deposited in Nicotiana pollen tube walls. Expression of several xyloglucan-related genes in actively growing pollen tubes was confirmed and xyloglucan epitopes were detected in the wall with carbohydrate-specific antibodies: the major xyloglucan oligosaccharides found in N. alata pollen grains and tubes were fucosylated, an unusual structure for the Solanaceae, the family to which Nicotiana belongs. Finally, carbohydrate linkages consistent with xyloglucan were identified chemically in the walls of N. alata pollen grains and pollen tubes grown in culture. The presence of a fucosylated xyloglucan in Nicotiana pollen tube walls was thus confirmed. The consequences of this discovery to models of pollen tube growth dynamics and more generally to polarised tip-growing cells in plants are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.