ArticlePloS one2013
Volatile emissions from Mycobacterium avium subsp. paratuberculosis mirror bacterial growth and enable distinction of different strains.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Pooled it
- Detection ofSensors (Basel, Switzerland) · 2024Article
- Volatile organic compound detection of Buruli ulcer disease: Headspace analysis of Mycobacterium ulcerans and used gauzes of Buruli-compatible ulcers.PLoS neglected tropical diseases · 2024Article
- Breath metabolomics for diagnosis of acute respiratory distress syndrome.Critical care (London, England) · 2024Observational
- Thiol Metabolism and Volatile Metabolome ofFrontiers in microbiology · 2022Article
- (S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds.International journal of molecular sciences · 2021Article
- MAP, Johne's disease and the microbiome; current knowledge and future considerations.Animal microbiome · 2021Review
- Volatile Organic Compounds, Bacterial Airway Microbiome, Spirometry and Exercise Performance of Patients after Surgical Repair of Congenital Diaphragmatic Hernia.Molecules (Basel, Switzerland) · 2021Article
- Detection ofFrontiers in veterinary science · 2021Article
- Application of Volatilome Analysis to the Diagnosis of Mycobacteria Infection in Livestock.Frontiers in veterinary science · 2021Review
- Screening of Microbial Volatile Organic Compounds for Detection of Disease in Cattle: Development of Lab-scale Method.Scientific reports · 2019Article
- Article
- Article
- Comparative analysis of volatile organic compounds for the classification and identification of mycobacterial species.PloS one · 2018Article
- Enhanced non-invasive respiratory sampling from bottlenose dolphins for breath metabolomics measurements.Journal of breath research · 2016Article
- Genetic diversity of Mycobacterium avium subspecies paratuberculosis and the influence of strain type on infection and pathogenesis: a review.Veterinary research · 2015Review
- Volatile-sulfur-compound profile distinguishes Burkholderia pseudomallei from Burkholderia thailandensis.Journal of clinical microbiology · 2015Article
- In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.PloS one · 2015Article
- Diversity and functions of volatile organic compounds produced by Streptomyces from a disease-suppressive soil.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Control of paratuberculosis in livestock is hampered by the low sensitivity of established direct and indirect diagnostic methods. Like other bacteria, Mycobacterium avium subsp. paratuberculosis (MAP) emits volatile organic compounds (VOCs). Differences of VOC patterns in breath and feces of infected and not infected animals were described in first pilot experiments but detailed information on potential marker substances is missing. This study was intended to look for characteristic volatile substances in the headspace of cultures of different MAP strains and to find out how the emission of VOCs was affected by density of bacterial growth. One laboratory adapted and four field strains, three of MAP C-type and one MAP S-type were cultivated on Herrold's egg yolk medium in dilutions of 10(-0), 10(-2), 10(-4) and 10(-6). Volatile substances were pre-concentrated from the headspace over the MAP cultures by means of Solid Phase Micro Extraction (SPME), thermally desorbed from the SPME fibers and separated and identified by means of GC-MS. Out of the large number of compounds found in the headspace over MAP cultures, 34 volatile marker substances could be identified as potential biomarkers for growth and metabolic activity. All five MAP strains could clearly be distinguished from blank culture media by means of emission patterns based on these 34 substances. In addition, patterns of volatiles emitted by the reference strain were significantly different from the field strains. Headspace concentrations of 2-ethylfuran, 2-methylfuran, 3-methylfuran, 2-pentylfuran, ethyl acetate, 1-methyl-1-H-pyrrole and dimethyldisulfide varied with density of bacterial growth. Analysis of VOCs emitted from mycobacterial cultures can be used to identify bacterial growth and, in addition, to differentiate between different bacterial strains. VOC emission patterns may be used to approximate bacterial growth density. In a perspective volatile marker substances could be used to diagnose MAP infections in animals and to identify different bacterial strains and origins.
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