ArticleJournal of lipid research2013
Novel technique for generating macrophage foam cells for in vitro reverse cholesterol transport studies.
Article in Journal of lipid research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- The cardioprotective role of the G protein-coupled receptor FFAR4 in atherosclerosis is independent of macrophage foam cell regulation.The Journal of biological chemistry · 2025Article
- Isochlorogenic Acid C Alleviates High-Fat Diet-Induced Hyperlipemia by Promoting Cholesterol Reverse Transport.Frontiers in pharmacology · 2022Article
- Preparation of LDL , Oxidation , Methods of Detection, and Applications in Atherosclerosis Research.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Apoptotic cell death induced by dendritic derivatives of aminolevulinic acid in endothelial and foam cells co-cultures.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Advances in Cardiovascular Disease Lipid Research Can Provide Novel Insights Into Mycobacterial Pathogenesis.Frontiers in cellular and infection microbiology · 2019Review
- Quantification of sterol-specific response in human macrophages using automated imaged-based analysis.Lipids in health and disease · 2017Article
- Anti-atherosclerotic effect of traditional fermented cheese whey in atherosclerotic rabbits and identification of probiotics.BMC complementary and alternative medicine · 2016Article
- Differential lipid metabolism in monocytes and macrophages: influence of cholesterol loading.Journal of lipid research · 2016Article
- Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease.Physiological reports · 2016Article
- Impaired cardiac energy metabolism in embryos lacking adrenergic stimulation.American journal of physiology. Endocrinology and metabolism · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generation of foam cells, an essential step for reverse cholesterol transport studies, uses the technique of receptor-dependent macrophage loading with radiolabeled acetylated LDL. In this study, we used the ability of a biologically relevant detergent molecule, lysophosphatidylcholine (lyso-PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabeled cholesterol/lyso-PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month of storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled, or radiolabeled)/lyso-PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by TLC. Such foam cells unloaded cholesterol when incubated with HDL but not with oxidized HDL. We propose that stable cholesterol or CE/lyso-PtdCho micelles would offer advantages over existing methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.