ArticleThe Journal of biological chemistry2013
Glycerophosphocholine utilization by Candida albicans: role of the Git3 transporter in virulence.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
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- How Not to be Seen: Predicting Unseen Enzyme Functions using Contrastive Learning.bioRxiv : the preprint server for biology · 2026Article
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- Cocrystal-inspired glycyrrhizic acid-azole nanoassemblies for synergistic biofilm disruption and immune modulation in fungal infections.Journal of nanobiotechnology · 2026Article
- Recent perspectives on biotechnological production, modulation and applications of glycerophosphoryl diester phosphodiesterases.Biodegradation · 2025Review
- Candida albicans' inorganic phosphate transport and evolutionary adaptation to phosphate scarcity.PLoS genetics · 2024Article
- An update on Glycerophosphodiester Phosphodiesterases; From Bacteria to Human.The protein journal · 2024Review
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- Glycerophosphocholine provision rescuesmSphere · 2023Article
- In vivo detection of dysregulated choline metabolism in paclitaxel-resistant ovarian cancers with proton magnetic resonance spectroscopy.Journal of translational medicine · 2022Article
- Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization.The Crop journal · 2021Article
- Human GDPD3 overexpression promotes liver steatosis by increasing lysophosphatidic acid production and fatty acid uptake.Journal of lipid research · 2020Article
- Phosphate in Virulence ofJournal of fungi (Basel, Switzerland) · 2020Review
- Genetically Compromising Phospholipid Metabolism Limits Candida albicans' Virulence.Mycopathologia · 2019Article
- White-to-opaque switching is involved in the phospholipase B production of Candida dubliniensis on Price's egg yolk agar.Odontology · 2019Article
- Genome-Wide Signatures of Selection inFrontiers in microbiology · 2019Article
- Overproduction of Phospholipids by the Kennedy Pathway Leads to Hypervirulence inFrontiers in microbiology · 2019Article
- Phosphate Acquisition and Virulence in Human Fungal Pathogens.Microorganisms · 2017Review
- Cloning of Glycerophosphocholine Acyltransferase (GPCAT) from Fungi and Plants: A NOVEL ENZYME IN PHOSPHATIDYLCHOLINE SYNTHESIS.The Journal of biological chemistry · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Candida albicans contains four ORFs (GIT1,2,3,4) predicted to encode proteins involved in the transport of glycerophosphodiester metabolites. Previously, we reported that Git1, encoded by ORF 19.34, is responsible for the transport of intact glycerophosphoinositol but not glycerophosphocholine (GroPCho). Here, we report that a strain lacking both GIT3 (ORF 19.1979) and GIT4 (ORF 19.1980) is unable to transport [(3)H]GroPCho into the cell. In the absence of a GroPCho transporter, C. albicans can utilize GroPCho via a mechanism involving extracellular hydrolysis. Upon reintegration of either GIT3 or GIT4 into the genome, measurable uptake of [(3)H]GroPCho is observed. Transport assays and kinetic analyses indicate that Git3 has the greater transport velocity. We present evidence that GDE1 (ORF 19.3936) codes for an enzyme with glycerophosphodiesterase activity against GroPCho. Homozygous deletion of GDE1 results in a buildup of internal GroPCho that is restored to wild type levels by reintegration of GDE1 into the genome. The transcriptional regulator, Pho4, is shown to regulate the expression of GIT3, GIT4, and GDE1. Finally, Git3 is shown to be required for full virulence in a mouse model of disseminated candidiasis, and Git3 sequence orthologs are present in other pathogenic Candida species. In summary, we have characterized multiple aspects of GroPCho utilization by C. albicans and have demonstrated that GroPCho transport plays a key role in the growth of the organism in the host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.