Trial reportThe Journal of biological chemistry2013
17β-Estradiol inhibits phosphorylation of stromal interaction molecule 1 (STIM1) protein: implication for store-operated calcium entry and chronic lung diseases.
Trial report in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Is the SPLUNC1-Orai1 axis a critical determinant of lung health?Biochemical Society transactions · 2025Review
- Air-Liquid interface cultures to model drug delivery through the mucociliary epithelial barrier.Advanced drug delivery reviews · 2023Review
- Estrogenic Modulation of Ionic Channels, Pumps and Exchangers in Airway Smooth Muscle.International journal of molecular sciences · 2023Review
- Chronic Obstructive Pulmonary Disease: Novel Genes Detection with Penalized Logistic Regression.Cell journal · 2023Article
- Super resolution microscopy analysis reveals increased Orai1 activity in asthma and cystic fibrosis lungs.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2023Article
- Hormonal Effects on Asthma, Rhinitis, and Eczema.The journal of allergy and clinical immunology. In practice · 2022Review
- Sex, Cells, and Asthma.Mayo Clinic proceedings · 2021Review
- Gender-Dependent Phenotype in Polycystic Kidney Disease Is Determined by Differential Intracellular CaInternational journal of molecular sciences · 2021Article
- Large pH oscillations promote host defense against human airways infection.The Journal of experimental medicine · 2021Article
- Effects of estrogen on STIM1/Orai1 in the sublingual gland of ovariectomized rats.Histology and histopathology · 2020Article
- Flavored e-liquids increase cytoplasmic CaAmerican journal of physiology. Lung cellular and molecular physiology · 2020Article
- Reciprocality Between Estrogen Biology and Calcium Signaling in the Cardiovascular System.Frontiers in endocrinology · 2020Review
- The steroid hormone 20-hydroxyecdysone induces phosphorylation and aggregation of stromal interacting molecule 1 for store-operated calcium entry.The Journal of biological chemistry · 2019Article
- Diverse actions of estradiol on anorexigenic and orexigenic hypothalamic arcuate neurons.Hormones and behavior · 2018Review
- Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome.American journal of respiratory and critical care medicine · 2018Article
- Gender differences in bronchiectasis: a real issue?Breathe (Sheffield, England) · 2018Review
- SPLUNC1 is an allosteric modulator of the epithelial sodium channel.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance.Endocrinology · 2018Article
- Identification of BPIFA1/SPLUNC1 as an epithelium-derived smooth muscle relaxing factor.Nature communications · 2017Article
- G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca(2+) signaling pathway in human airway epithelia.Pflugers Archiv : European journal of physiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Sex plays a significant role in the development of lung diseases including asthma, cancer, chronic bronchitis, and cystic fibrosis. In cystic fibrosis, 17β-estradiol (E2) may inhibit store-operated Ca(2+) entry (SOCE) to impinge upon airway secretions, leaving females at greater risk of contracting lung infections. Stromal interaction molecule 1 (STIM1)-mediated SOCE is essential for cell homeostasis and regulates numerous processes including cell proliferation, smooth muscle contraction, and secretion. E2 can signal nongenomically to modulate Ca(2+) signaling, but little is known of the underlying mechanisms. We found that E2 exposure inhibited STIM1 translocation in airway epithelia, preventing SOCE. This correlated with a decrease in STIM1-STIM1 FRET and STIM1 mobility in E2-exposed HEK293T cells co-expressing estrogen receptor α. We also examined the role of STIM1 phosphorylation in E2-mediated inhibition of STIM1 mobility. STIM1 is basally phosphorylated at serine 575, which is required for SOCE. Exposure to E2 significantly decreased STIM1 serine phosphorylation. Mutating serine 575 to an alanine blocked STIM1 phosphorylation, reduced basal STIM1 mobility, and rendered STIM1 insensitive to E2. These data indicate that E2 can signal nongenomically by inhibiting basal phosphorylation of STIM1, leading to a reduction in SOCE.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.