ArticleScience (New York, N.Y.)2013
Evidence that the fosfomycin-producing epoxidase, HppE, is a non-heme-iron peroxidase.
Article in Science (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 76 citations in OpenAlex.
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- Influence of Primary Coordination Sphere on Anion Rebound Selectivity in Nonheme Fe Enzyme-Catalyzed C(spbioRxiv : the preprint server for biology · 2026Article
- The curious life of human mitochondrial SOD2.International journal of biological macromolecules · 2026Review
- Substrate Epoxidation Catalyzed by the Nonheme Iron Dioxygenase Dapdiamide Biosynthesis Enzyme C. Why Is the Substrate Tethered?Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Engineering non-haem iron enzymes for enantioselective C(sp3)-F bond formation via radical fluorine transfer.Nature synthesis · 2024Article
- Expanding the catalytic landscape of metalloenzymes with lytic polysaccharide monooxygenases.Nature reviews. Chemistry · 2024Review
- Three-membered ring formation catalyzed by α-ketoglutarate-dependent nonheme iron enzymes.Journal of natural medicines · 2024Review
- Lytic polysaccharide monooxygenases: enzymes for controlled and site-specific Fenton-like chemistry.Essays in biochemistry · 2023Article
- Convergent and divergent biosynthetic strategies towards phosphonic acid natural products.Current opinion in chemical biology · 2022Review
- An Unusual Oxidative Rearrangement Catalyzed by a Divergent Member of the 2-Oxoglutarate-Dependent Dioxygenase Superfamily during Biosynthesis of Dehydrofosmidomycin.Angewandte Chemie (International ed. in English) · 2022Article
- Harnessing phosphonate antibiotics argolaphos biosynthesis enables a synthetic biology-based green synthesis of glyphosate.Nature communications · 2022Article
- Effect of the Rare Earth Element Lanthanum (La) on the Growth and Development of Citrus Rootstock Seedlings.Plants (Basel, Switzerland) · 2021Article
- Overall Retention of Methyl Stereochemistry during BBiochemistry · 2021Article
- Kinetic insights into the peroxygenase activity of cellulose-active lytic polysaccharide monooxygenases (LPMOs).Nature communications · 2020Article
- Nature's Machinery, Repurposed: Expanding the Repertoire of Iron-Dependent Oxygenases.ACS catalysis · 2020Article
- Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
- Epoxidation Catalyzed by the Nonheme Iron(II)- and 2-Oxoglutarate-Dependent Oxygenase, AsqJ: Mechanistic Elucidation of Oxygen Atom Transfer by a Ferryl Intermediate.Journal of the American Chemical Society · 2020Article
- Steric Enforcement ofJournal of the American Chemical Society · 2019Article
- The intriguing biology and chemistry of fosfomycin: the only marketed phosphonate antibiotic.RSC advances · 2019Review
- Catalase Involved in Oxidative Cyclization of the Tetracyclic Ergoline of Fungal Ergot Alkaloids.Journal of the American Chemical Society · 2019Article
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10 authors at 1 institution in 1 country.
Funding
Abstract
The iron-dependent epoxidase HppE converts (S)-2-hydroxypropyl-1-phosphonate (S-HPP) to the antibiotic fosfomycin [(1R,2S)-epoxypropylphosphonate] in an unusual 1,3-dehydrogenation of a secondary alcohol to an epoxide. HppE has been classified as an oxidase, with proposed mechanisms differing primarily in the identity of the O2-derived iron complex that abstracts hydrogen (H•) from C1 of S-HPP to initiate epoxide ring closure. We show here that the preferred cosubstrate is actually H2O2 and that HppE therefore almost certainly uses an iron(IV)-oxo complex as the H• abstractor. Reaction with H2O2 is accelerated by bound substrate and produces fosfomycin catalytically with a stoichiometry of unity. The ability of catalase to suppress the HppE activity previously attributed to its direct utilization of O2 implies that reduction of O2 and utilization of the resultant H2O2 were actually operant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.