ArticleInvestigative ophthalmology & visual science2013
Functional principal component analysis reveals discriminating categories of retinal pigment epithelial morphology in mice.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Spatio-temporal mechanosensitive phagocytic response of retinal pigment epithelial cells to reversible changes in substrate stiffness.Acta biomaterialia · 2026Article
- Stress Inference in Retinal Pigment Epithelium in C57BL/6J Mouse.Investigative ophthalmology & visual science · 2026Article
- High-Resolution Imaging and Interpretation of Three-Dimensional RPE Sheet Structure.Biomolecules · 2025Article
- Age-Related RPE changes in Wildtype C57BL/6J Mice between 2 and 32 Months.bioRxiv : the preprint server for biology · 2024Article
- Pentosan Polysulfate Sodium Causes Diminished Function and Subtle Morphological Changes in Retina and RPE of Mice.Investigative ophthalmology & visual science · 2024Article
- A New Preclinical Model of Retinitis Pigmentosa Due toOphthalmology science · 2023Article
- Self-supervised semantic segmentation of retinal pigment epithelium cells in flatmount fluorescent microscopy images.Bioinformatics (Oxford, England) · 2023Article
- Article
- Sensitivity of the Dorsal-Central Retinal Pigment Epithelium to Sodium Iodate-Induced Damage Is Associated With Overlying M-Cone Photoreceptors in Mice.Investigative ophthalmology & visual science · 2022Article
- Histologic Cell Shape Descriptors for the Retinal Pigment Epithelium in Age-Related Macular Degeneration: A Comparison to Unaffected Eyes.Translational vision science & technology · 2022Article
- Computational Model-Based Estimation of Mouse Eyeball Structure From Two-Dimensional Flatmount Microscopy Images.Translational vision science & technology · 2021Article
- Morphometric Analysis of Retinal Pigment Epithelial Cells From C57BL/6J Mice During Aging.Investigative ophthalmology & visual science · 2021Article
- Article
- Quantifying Retinal Pigment Epithelium Dysmorphia and Loss of Histologic Autofluorescence in Age-Related Macular Degeneration.Investigative ophthalmology & visual science · 2019Article
- Analysis of Damage and Wound Healing in the Retinal Pigmented Epithelium.Advances in experimental medicine and biology · 2019Article
- Wheel running exercise protects against retinal degeneration in the I307N rhodopsin mouse model of inducible autosomal dominant retinitis pigmentosa.Molecular vision · 2019Article
- Comparison of histologic findings in age-related macular degeneration with RPE flatmount images.Molecular vision · 2019Article
- Retinal pigment epithelial integrity is compromised in the developing albino mouse retina.The Journal of comparative neurology · 2016Article
- Texture Descriptors Ensembles Enable Image-Based Classification of Maturation of Human Stem Cell-Derived Retinal Pigmented Epithelium.PloS one · 2016Article
- RPE Cell and Sheet Properties in Normal and Diseased Eyes.Advances in experimental medicine and biology · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo determine whether multivariate, functional principal component analysis of the size and shape of retinal pigment epithelial (RPE) cell morphology allows discrimination of mouse RPE genotypes and age.
methodsFlatmounts of RPE sheets obtained from C57BL/6J (n = 50) and rd10 (n = 61) mice at postnatal days 30 to 720 were stained for zonula occludens-1 (ZO-1) and imaged with confocal microscopy. A total of 111 flatmounts were prepared. Twenty-one morphometric measurements were made on tiled, composite images of complete flatmounts, including cell location, area, and eccentricity, using automated image analysis software for quantitatively measuring cell phenotypes.
resultsIn young (≤61-day-old) C57BL/6J mice, the RPE morphology resembled a regular hexagonal array of cells of uniform size throughout the retina, except near the ciliary body, where the shapes of RPE resembled a soft network. Old (≥180-day-old) C57BL/6J eyes had a subpopulation of large cells. A clear disruption of the regular cell size and shape appeared in rd10 mutants. Aspect ratio and cell area gave rise to principal components that predictively classified mouse age and genotype.
conclusionsQuantitative differences in the RPE sheet morphology allowed discrimination of rd10 from C57BL/6J strains despite the confounding effect of aging. This has implications for RPE sheet morphology as a potential early biomarker for diagnosis of eye disease and prognosis of the eye at early stages when disease is subtle. We conclude that an RPE cell's area and aspect ratio are very early quantitative indicators that predict progression to advanced RPE disease as manifested in rd10.
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