ArticleOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA2014
Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture.
Article in Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Identification of osteoclast-osteoblast coupling factors in humans reveals links between bone and energy metabolism.Nature communications · 2020Trial
- Effects of estrogen on bone mRNA levels of sclerostin and other genes relevant to bone metabolism in postmenopausal women.The Journal of clinical endocrinology and metabolism · 2014Trial
- Dissection of Cellular Communication between Human Primary Osteoblasts and Bone Marrow Mesenchymal Stem Cells in Osteoarthritis at Single-Cell Resolution.International journal of stem cells · 2023Article
- From the Destruction of Two Lumbar Segments to Thoracic-Lumbar-Pelvic Fusion: A Case Caused by Congenital Insensitivity to Pain with Anhidrosis and Literature Review.Orthopaedic surgery · 2023Review
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- Human cells with osteogenic potential in bone tissue research.Biomedical engineering online · 2023Review
- Isolating mineralized bone and bone marrow mRNA from transiliac bone biopsies stored in a stabilizing solution: A comparative study.Bone reports · 2022Article
- Imputation of Human Primary Osteoblast Single Cell RNA-Seq Data Identified Three Novel Osteoblastic Subtypes.Frontiers in bioscience (Landmark edition) · 2022Article
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- Osteogenic transdifferentiation of primary human fibroblasts to osteoblast-like cells with human platelet lysate.Scientific reports · 2022Article
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- Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques.Frontiers in endocrinology · 2021Review
- Independent Roles of Estrogen Deficiency and Cellular Senescence in the Pathogenesis of Osteoporosis: Evidence in Young Adult Mice and Older Humans.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes.Bone reports · 2019Article
- Osteogenesis depends on commissioning of a network of stem cell transcription factors that act as repressors of adipogenesis.Nature genetics · 2019Article
- Ascorbic Acid Attenuates Senescence of Human Osteoarthritic Osteoblasts.International journal of molecular sciences · 2017Article
- Identification of Senescent Cells in the Bone Microenvironment.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
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- Effects of Age and Estrogen on Skeletal Gene Expression in Humans as Assessed by RNA Sequencing.PloS one · 2015Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
summaryWe isolate and characterize osteoblasts from humans without in vitro culture. These techniques should be broadly applicable to studying the pathogenesis of osteoporosis and other bone disorders.
introductionThere is currently no data regarding the expression of specific genes or pathways in human osteoblasts that have not been subjected to extensive in vitro culture. Thus, we developed methods to rapidly isolate progressively enriched osteoblast populations from humans and characterized these cells.
methodsNeedle bone biopsies of the posterior iliac crest were subjected to sequential collagenase digests. The cells from the second digest were stained with an alkaline phosphatase (AP) antibody, and the AP+ cells were isolated using magnetic cell sorting.
resultsRelative to AP- cells, the AP+ cells contained virtually all of the mineralizing cells and were enriched for key osteoblast marker genes. The AP+ cells were further purified by depletion of cells expressing CD45, CD34, or CD31 (AP+/CD45/34/31- cells), which represented a highly enriched human osteoblast population devoid of hematopoietic/endothelial cells. These cells expressed osteoblast marker genes but very low to undetectable levels of SOST. We next used high-throughput RNA sequencing to compare the transcriptome of the AP+/CD45/34/31- cells to human fibroblasts and identified genes and pathways expressed only in human osteoblasts in vivo, but not in fibroblasts, including 448 genes unique to human osteoblasts.
conclusionsWe provide a detailed characterization of highly enriched human osteoblast populations without in vitro culture. These techniques should be broadly applicable to studying the pathogenesis of osteoporosis and other bone disorders.
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