ArticleCytotechnology2014
Primary culture of human face skin melanocytes for the study of hyperpigmentation.
Article in Cytotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Horizontal mitochondrial transfer and mitochondrial transplantation in skin: protection against UVR-induced ROS damage and enhancement of cell proliferation and wound healing.Journal of translational medicine · 2026Article
- Multiple Stem/Progenitor Cells Isolated from the Limbus.Current stem cell research & therapy · 2025Review
- Differentiation of cultured hair follicle neural crest stem cells into functional melanocytes.Heliyon · 2024Article
- Multiple gene-drug prediction tool reveals Rosiglitazone based treatment pathway for non-segmental vitiligo.Inflammation · 2024Article
- The story of melanocyte: a long way from bench to bedside.Cell and tissue banking · 2024Review
- Melanogrit potentiates melanogenesis by escalating cellular tyrosinase activity and MITF levels via pERK inhibition.Bioscience reports · 2024Article
- Effect of Commercial Children's Mouthrinses and Toothpastes on the Viability of Neonatal Human Melanocytes: An In Vitro Study.Dentistry journal · 2023Article
- Review
- Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders.International journal of molecular sciences · 2023Article
- Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin.Bio-protocol · 2022Article
- Canine Epidermal Keratinocytes (CPEK) Grown in Monolayer Are Not Representative of Normal Canine Keratinocytes for Permeability Studies: Pilot Studies.Veterinary sciences · 2022Article
- Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes.Animals : an open access journal from MDPI · 2021Article
- Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.Cell and tissue banking · 2021Article
- Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker.Scientific reports · 2020Article
- Development and validation of a simple method for the extraction of human skin melanocytes.Cytotechnology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Facial epidermal pigmentation and skin tumors can be caused by UV exposure and other physical and chemical irritations. In this report we describe the primary culture of melanocytes from human face skin. The ability to culture these melanocytes will enable their morphological and biological properties to be investigated. Skin specimens were obtained from patients who had undergone lower blepharoplasty procedures. Digestion with neutral protease and trypsin was used to obtain single cell suspensions of epidermal cells. The cells were cultured in M254 medium supplemented with human melanocyte growth solution. Cell morphology was observed using inverted microscopy. Melanocytes were positively identified using both L-DOPA staining and S-100 protein immunohistochemical staining. Immunofluorescence was used to confirm the expression of tyrosinase-related protein-1, a melanocyte-specific protein. The cellular ultrastructure of the melanocytes was observed by transmission electron microscopy. The cultured human melanocytes from face skin were multi-dendritic, and many mature melanosomes were observed. Therefore, using a specific culture medium, melanocytes from face skin can be successfully cultured and made available for further investigations.
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Registered trials
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