ArticleJournal of industrial microbiology & biotechnology2014
Enhanced expression of genes involved in initial xylose metabolism and the oxidative pentose phosphate pathway in the improved xylose-utilizing Saccharomyces cerevisiae through evolutionary engineering.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Lignocellulosic biomass fermentation: a roadmap for Candida famata and Ogataea polymorpha.FEMS yeast research · 2025Review
- Integration of metabolism and regulation reveals rapid adaptability to growth on non-native substrates.Cell chemical biology · 2023Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene.Microbial cell factories · 2022Article
- Deletion ofFrontiers in microbiology · 2022Article
- Microbial Adaptation to Enhance Stress Tolerance.Frontiers in microbiology · 2022Review
- D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain.FEMS yeast research · 2021Article
- Yeast-Based Biosynthesis of Natural Products From Xylose.Frontiers in bioengineering and biotechnology · 2021Review
- Different transcriptional responses of haploid and diploid S. cerevisiae strains to changes in cofactor preference of XR.Microbial cell factories · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- Association of improved oxidative stress tolerance and alleviation of glucose repression with superior xylose-utilization capability by a natural isolate ofBiotechnology for biofuels · 2018Article
- Heterologous Biosynthesis of the Fungal Sesquiterpene Trichodermol inFrontiers in microbiology · 2018Article
- Process analysis and optimization of simultaneous saccharification and co-fermentation of ethylenediamine-pretreated corn stover for ethanol production.Biotechnology for biofuels · 2018Article
- Improved Xylose Metabolism by aApplied and environmental microbiology · 2017Article
- Fungal-mediated consolidated bioprocessing: the potential of Fusarium oxysporum for the lignocellulosic ethanol industry.AMB Express · 2016Article
- Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Enhanced Bioconversion of Cellobiose by Industrial Saccharomyces cerevisiae Used for Cellulose Utilization.Frontiers in microbiology · 2016Article
- Ethylenediamine pretreatment changes cellulose allomorph and lignin structure of lignocellulose at ambient pressure.Biotechnology for biofuels · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fermentation of xylose in lignocellulosic hydrolysates by Saccharomyces cerevisiae has been achieved through heterologous expression of the xylose reductase (XR)-xylitol dehydrogenase (XDH) pathway. However, the fermentation efficiency is far from the requirement for industrial application due to high yield of the byproduct xylitol, low ethanol yield, and low xylose consumption rate. Through evolutionary engineering, an improved xylose-utilizing strain SyBE005 was obtained with 78.3 % lower xylitol production and a 2.6-fold higher specific ethanol production rate than those of the parent strain SyBE004, which expressed an engineered NADP(+)-preferring XDH. The transcriptional differences between SyBE005 and SyBE004 were investigated by quantitative RT-PCR. Genes including XYL1, XYL2, and XKS1 in the initial xylose metabolic pathway showed the highest up-regulation in SyBE005. The increased expression of XYL1 and XYL2 correlated with enhanced enzymatic activities of XR and XDH. In addition, the expression level of ZWF1 in the oxidative pentose phosphate pathway increased significantly in SyBE005, indicating an elevated demand for NADPH from XR. Genes involved in the TCA cycle (LAT1, CIT1, CIT2, KGD1, KGD, SDH2) and gluconeogenesis (ICL1, PYC1) were also up-regulated in SyBE005. Genomic analysis revealed that point mutations in transcriptional regulators CYC8 and PHD1 might be responsible for the altered expression. In addition, a mutation (Y89S) in ZWF1 was identified which might improve NADPH production in SyBE005. Our results suggest that increasing the expression of XYL1, XYL2, XKS1, and enhancing NADPH supply are promising strategies to improve xylose fermentation in recombinant S. cerevisiae.
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