ArticleApplied microbiology and biotechnology2013
SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.
Article in Applied microbiology and biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- High-throughput multiplex qPCR for distinguishing Salmonella serovars Heidelberg, Enteritidis, Typhimurium, Gallinarum, and Pullorum.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Characterization of Salmonella species from poultry slaughterhouses in South Korea: carry-over transmission ofJournal of veterinary science · 2024Article
- Salmonella detection with LAMP and qPCR and identification of serovars of interest by multiplex qPCR in poultry carcasses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Differences between the global transcriptomes of Salmonella enterica serovars Dublin and Cerro infecting bovine epithelial cells.BMC genomics · 2022Article
- Newly developed SYBR Green-based quantitative real-time PCRs revealed coinfection evidence ofFood and waterborne parasitology · 2021Article
- Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of aMicrobial genomics · 2021Article
- Quantitative LAMP and PCR Detection ofInternational journal of food science · 2020Article
- Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.Applied microbiology and biotechnology · 2019Article
- High expression of type I inositol 1,4,5-trisphosphate receptor in the kidney of rats with hepatorenal syndrome.World journal of gastroenterology · 2018Article
- Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system.Applied microbiology and biotechnology · 2018Article
- Presence and Persistence ofFrontiers in public health · 2018Review
- Visual detection technique for efficient screening and isolation of Salmonella based on a novel enrichment assay using chromatography membrane.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium.Applied microbiology and biotechnology · 2015Article
- Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.Applied microbiology and biotechnology · 2015Article
- Genome Sequence of the Salmonella enterica subsp. enterica Serovar Namur Strain 05-2929, Lacking the Salmonella Atypical Fimbrial Operon.Genome announcements · 2014Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well as the STM0296 locus, putatively coding for a cytoplasmic protein. This study includes the design of primer pairs, in silico and in situ selectivity, sensitivity, repeatability and reproducibility evaluations of the seven SYBR®Green qPCR assays. Each detection level displayed a selectivity of 100 %. This combinatory SYBR®Green qPCR system was also compared with three commercially available Salmonella qPCR detection kits. This comparison highlighted the importance of using a multi-gene detection system to be able to detect every target strain, even those with deletion or mutation of important genes.
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