ArticleEMBO reports2013
No need for a power stroke in ISWI-mediated nucleosome sliding.
Article in EMBO reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Remodeling of Individual Nucleosomes in Nucleosome Arrays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.Nature structural & molecular biology · 2024Article
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.bioRxiv : the preprint server for biology · 2023Article
- Biophysics of Chromatin Remodeling.Annual review of biophysics · 2021Review
- Remodeler Catalyzed Nucleosome Repositioning: Influence of Structure and Stability.International journal of molecular sciences · 2020Review
- A basic motif anchoring ISWI to nucleosome acidic patch regulates nucleosome spacing.Nature chemical biology · 2020Article
- Molecular basis of chromatin remodeling by Rhp26, a yeast CSB ortholog.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.Nature reviews. Molecular cell biology · 2017Review
- Article
- Review
- Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.The Journal of biological chemistry · 2016Article
- Mechanisms of ATP-Dependent Chromatin Remodeling Motors.Annual review of biophysics · 2016Review
- Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density.Molecular and cellular biology · 2015Article
- A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.Molecular cell · 2015Article
- ISWI remodelling of physiological chromatin fibres acetylated at lysine 16 of histone H4.PloS one · 2014Article
- Remodelling without a power stroke.EMBO reports · 2013Article
- Nucleosome sliding by Chd1 does not require rigid coupling between DNA-binding and ATPase domains.EMBO reports · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleosome remodelling enzymes of the ISWI family reposition nucleosomes in eukaryotes. ISWI contains an ATPase and a HAND-SANT-SLIDE (HSS) domain. Conformational changes between these domains have been proposed to be critical for nucleosome repositioning by pulling flanking DNA into the nucleosome. We inserted flexible linkers at strategic sites in ISWI to disrupt this putative power stroke and assess its functional importance by quantitative biochemical assays. Notably, the flexible linkers did not disrupt catalysis. Instead of engaging in a power stroke, the HSS module might therefore assist DNA to ratchet into the nucleosome. Our results clarify the roles had by the domains and suggest that the HSS domain evolved to optimize a rudimentary remodelling engine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.