ArticleMolecular biology of the cell2013
NuMA localization, stability, and function in spindle orientation involve 4.1 and Cdk1 interactions.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 91 citations in OpenAlex.
- Activation and regulation of the dynein-dynactin-NuMA complex.Nature chemical biology · 2026Article
- Aurora A regulates the material property of spindle poles to orchestrate nuclear organization at mitotic exit.The EMBO journal · 2025Article
- Localized Wnt-signaling promotes asymmetric NuMA-dependent oriented divisions and unequal apportioning of mitochondria.Nature communications · 2025Article
- NuMA is a mitotic adaptor protein that activates dynein and connects it to microtubule minus ends.The Journal of cell biology · 2025Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.bioRxiv : the preprint server for biology · 2023Article
- Combined prediction and design reveals the target recognition mechanism of an intrinsically disordered protein interaction domain.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cortical dynein drives centrosome clustering in cells with centrosome amplification.Molecular biology of the cell · 2023Article
- AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.eLife · 2023Article
- The Drosophila mitotic spindle orientation machinery requires activation, not just localization.EMBO reports · 2023Article
- Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation.The Journal of cell biology · 2022Article
- Sculpting an Embryo: The Interplay between Mechanical Force and Cell Division.Journal of developmental biology · 2022Review
- Cortical Cyclin A controls spindle orientation during asymmetric cell divisions in Drosophila.Nature communications · 2022Article
- The importance of the compact disordered state in the fuzzy interactions between intrinsically disordered proteins.Chemical science · 2022Article
- Insights Into Mechanisms of Oriented Division From Studies in 3D Cellular Models.Frontiers in cell and developmental biology · 2022Review
- KIF18B is a cell type-specific regulator of spindle orientation in the epidermis.Molecular biology of the cell · 2021Article
- Spindle positioning and its impact on vertebrate tissue architecture and cell fate.Nature reviews. Molecular cell biology · 2021Review
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.EMBO reports · 2021Article
- 4.1N-Mediated Interactions and Functions in Nerve System and Cancer.Frontiers in molecular biosciences · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The epidermis is a multilayered epithelium that requires asymmetric divisions for stratification. A conserved cortical protein complex, including LGN, nuclear mitotic apparatus (NuMA), and dynein/dynactin, plays a key role in establishing proper spindle orientation during asymmetric divisions. The requirements for the cortical recruitment of these proteins, however, remain unclear. In this work, we show that NuMA is required to recruit dynactin to the cell cortex of keratinocytes. NuMA's cortical recruitment requires LGN; however, LGN interactions are not sufficient for this localization. Using fluorescence recovery after photobleaching, we find that the 4.1-binding domain of NuMA is important for stabilizing its interaction with the cell cortex. This is functionally important, as loss of 4.1/NuMA interaction results in spindle orientation defects, using two distinct assays. Furthermore, we observe an increase in cortical NuMA localization as cells enter anaphase. Inhibition of Cdk1 or mutation of a single residue in NuMA mimics this effect. NuMA's anaphase localization is independent of LGN and 4.1 interactions, revealing two distinct mechanisms responsible for NuMA cortical recruitment at different stages of mitosis. This work highlights the complexity of NuMA localization and reveals the importance of NuMA cortical stability for productive force generation during spindle orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.