ArticleFrontiers in microbiology2013
Design of a large-scale femtoliter droplet array for single-cell analysis of drug-tolerant and drug-resistant bacteria.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- MALDI Mass Spectrometry on High-Density Droplet Arrays: Matrix Deposition, Selective Removal, and Recrystallization.ACS measurement science au · 2024Article
- Recent Advances in Bacterial Persistence Mechanisms.International journal of molecular sciences · 2023Review
- Studying Bacterial Persistence: Established Methods and Current Advances.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Complete inclusion of bioactive molecules and particles in polydimethylsiloxane: a straightforward process under mild conditions.Scientific reports · 2019Article
- Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.Analytical chemistry · 2018Article
- Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.SLAS technology · 2017Article
- Microfluidics for Antibiotic Susceptibility and Toxicity Testing.Bioengineering (Basel, Switzerland) · 2016Review
- Distinguishing between resistance, tolerance and persistence to antibiotic treatment.Nature reviews. Microbiology · 2016Review
- A Microfluidic Channel Method for Rapid Drug-Susceptibility Testing of Pseudomonas aeruginosa.PloS one · 2016Article
- Aminoglycoside-enabled elucidation of bacterial persister metabolism.Current protocols in microbiology · 2015Article
- Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa.Frontiers in microbiology · 2015Review
- Mechanisms of antibiotic resistance.Frontiers in microbiology · 2015Article
- Enzyme molecules in solitary confinement.Molecules (Basel, Switzerland) · 2014Review
- Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The role of metabolism in bacterial persistence.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell analysis is a powerful method to assess the heterogeneity among individual cells, enabling the identification of very rare cells with properties that differ from those of the majority. In this Methods Article, we describe the use of a large-scale femtoliter droplet array to enclose, isolate, and analyze individual bacterial cells. As a first example, we describe the single-cell detection of drug-tolerant persisters of Pseudomonas aeruginosa treated with the antibiotic carbenicillin. As a second example, this method was applied to the single-cell evaluation of drug efflux activity, which causes acquired antibiotic resistance of bacteria. The activity of the MexAB-OprM multidrug efflux pump system from Pseudomonas aeruginosa was expressed in Escherichia coli and the effect of an inhibitor D13-9001 were assessed at the single cell level.
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