ArticleJournal of virology2013
The Fc region of an antibody impacts the neutralization of West Nile viruses in different maturation states.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 20 citations in OpenAlex.
- West Nile virus unmasked: from gene variability to future challenges.Frontiers in cellular and infection microbiology · 2025Review
- pr-independent biogenesis of infectious mature Zika virus particles.bioRxiv : the preprint server for biology · 2024Article
- Implications of a highly divergent dengue virus strain for cross-neutralization, protection, and vaccine immunity.Cell host & microbe · 2021Article
- Experimental Assessment of Possible Factors Associated with Tick-Borne Encephalitis Vaccine Failure.Microorganisms · 2021Article
- Substitutions at Loop Regions of TMUV E Protein Domain III Differentially Impair Viral Entry and Assembly.Frontiers in microbiology · 2021Article
- Identification of Dengue Virus Serotype 3 Specific Antigenic Sites Targeted by Neutralizing Human Antibodies.Cell host & microbe · 2020Article
- Deconstructing the Antiviral Neutralizing-Antibody Response: Implications for Vaccine Development and Immunity.Microbiology and molecular biology reviews : MMBR · 2016Review
- Plasticity of a critical antigenic determinant in the West Nile virus NY99 envelope protein domain III.Virology · 2016Article
- Article
- A humanized monoclonal antibody neutralizes yellow fever virus strain 17D-204 in vitro but does not protect a mouse model from disease.Antiviral research · 2016Article
- A game of numbers: the stoichiometry of antibody-mediated neutralization of flavivirus infection.Progress in molecular biology and translational science · 2015Review
- Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition.Journal of virology · 2014Article
- The West Nile virus-like flavivirus Koutango is highly virulent in mice due to delayed viral clearance and the induction of a poor neutralizing antibody response.Journal of virology · 2014Article
- Particle conformation regulates antibody access to a conserved GII.4 norovirus blockade epitope.Journal of virology · 2014Article
- Review
- Dengue serotype cross-reactive, anti-e protein antibodies confound specific immune memory for 1 year after infection.Frontiers in immunology · 2014Article
- Article
- Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Flavivirus-infected cells secrete a structurally heterogeneous population of viruses because of an inefficient virion maturation process. Flaviviruses assemble as noninfectious, immature virions composed of trimers of envelope (E) and precursor membrane (prM) protein heterodimers. Cleavage of prM is a required process during virion maturation, although this often remains incomplete for infectious virus particles. Previous work demonstrated that the efficiency of virion maturation could impact antibody neutralization through changes in the accessibility of otherwise cryptic epitopes on the virion. In this study, we show that the neutralization potency of monoclonal antibody (MAb) E33 is sensitive to the maturation state of West Nile virus (WNV), despite its recognition of an accessible epitope, the domain III lateral ridge (DIII-LR). Comprehensive epitope mapping studies with 166 E protein DIII-LR variants revealed that the functional footprint of MAb E33 on the E protein differs subtly from that of the well-characterized DIII-LR MAb E16. Remarkably, aromatic substitutions at E protein residue 306 ablated the maturation state sensitivity of E33 IgG, and the neutralization efficacy of E33 Fab fragments was not affected by changes in the virion maturation state. We propose that E33 IgG binding on mature virions orients the Fc region in a manner that impacts subsequent antibody binding to nearby sites. This Fc-mediated steric constraint is a novel mechanism by which the maturation state of a virion modulates the efficacy of the humoral immune response to flavivirus infection.
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