ArticleNature2013
Visualizing virus assembly intermediates inside marine cyanobacteria.
Article in Nature, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 140 citations in OpenAlex.
- Electron cryotomography reveals ultrastructure alterations in platelets from patients with ovarian cancer.Proceedings of the National Academy of Sciences of the United States of America · 2015Trial
- BmNPV interacts with super-enhancer regions of the host chromatin to hijack cellular transcription machinery.Nucleic acids research · 2025Article
- Wiener filter unifies Hilbert and Zernike phase plates in electron microscopy.Biophysical reviews · 2025Review
- Visualizing the virus world inside the cell by cryo-electron tomography.Journal of virology · 2024Review
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Stable coexistence between an archaeal virus and the dominant methanogen of the human gut.Nature communications · 2024Article
- The application and development of electron microscopy for three-dimensional reconstruction in life science: a review.Cell and tissue research · 2024Review
- Conventional Electron Microscopy, Cryogenic Electron Microscopy, and Cryogenic Electron Tomography of Viruses.Sub-cellular biochemistry · 2024Review
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- Cryo-EM structure of cyanophage P-SCSP1u offers insights into DNA gating and evolution of T7-like viruses.Nature communications · 2023Article
- Cryo-electron tomography to study viral infection.Biochemical Society transactions · 2023Review
- Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene.The ISME journal · 2023Article
- Ot2Rec: A semi-automatic, extensible, multi-software tomographic reconstruction workflow.Biological imaging · 2023Article
- CryomicroscopyFaraday discussions · 2022Article
- Three-dimensional electron ptychography of organic-inorganic hybrid nanostructures.Nature communications · 2022Article
- Imaging biological macromolecules in thick specimens: The role of inelastic scattering in cryoEM.Ultramicroscopy · 2022Article
- Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.Science advances · 2022Article
- Viral Ejection Proteins: Mosaically Conserved, Conformational Gymnasts.Microorganisms · 2022Review
- Confocal microscopy reveals alterations of thylakoids in Limnospira fusiformis during prophage induction.Protoplasma · 2021Article
- Cryo-EM structure of the periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution.Molecular cell · 2021Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Cyanobacteria are photosynthetic organisms responsible for ∼25% of organic carbon fixation on the Earth. These bacteria began to convert solar energy and carbon dioxide into bioenergy and oxygen more than two billion years ago. Cyanophages, which infect these bacteria, have an important role in regulating the marine ecosystem by controlling cyanobacteria community organization and mediating lateral gene transfer. Here we visualize the maturation process of cyanophage Syn5 inside its host cell, Synechococcus, using Zernike phase contrast electron cryo-tomography (cryoET). This imaging modality yields dramatic enhancement of image contrast over conventional cryoET and thus facilitates the direct identification of subcellular components, including thylakoid membranes, carboxysomes and polyribosomes, as well as phages, inside the congested cytosol of the infected cell. By correlating the structural features and relative abundance of viral progeny within cells at different stages of infection, we identify distinct Syn5 assembly intermediates. Our results indicate that the procapsid releases scaffolding proteins and expands its volume at an early stage of genome packaging. Later in the assembly process, we detected full particles with a tail either with or without an additional horn. The morphogenetic pathway we describe here is highly conserved and was probably established long before that of double-stranded DNA viruses infecting more complex organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.