ArticleProceedings of the National Academy of Sciences of the United States of America2013
High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
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- Point spread function decoupling in computational fluorescence microscopy.Light, science & applications · 2026Article
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- Coding principles of dopaminergic transmission modes.Science advances · 2025Article
- Computational Super-Resolution: An Odyssey in Harnessing Priors to Enhance Optical Microscopy Resolution.Analytical chemistry · 2025Review
- Deconvolution to restore cryo-EM maps with anisotropic resolution.Research square · 2025Article
- Deconvolution to restore cryo-EM maps with anisotropic resolution.bioRxiv : the preprint server for biology · 2025Article
- DeepCristae, a CNN for the restoration of mitochondria cristae in live microscopy images.Communications biology · 2025Article
- Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.Journal of biomedical optics · 2024Article
- Pretraining a foundation model for generalizable fluorescence microscopy-based image restoration.Nature methods · 2024Article
- Cross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice.Nature methods · 2023Article
- Sidelobe suppression in structured light sheet fluorescence microscopy by the superposition of two light sheets.Biomedical optics express · 2023Article
- The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.Nature communications · 2023Article
- SPITFIR(e): a supermaneuverable algorithm for fast denoising and deconvolution of 3D fluorescence microscopy images and videos.Scientific reports · 2023Article
- Use of epifluorescence widefield deconvolution microscopy for imaging and three-dimensional rendering ofMethods in microbiology · 2023Article
- DARTS: an open-source Python pipeline for CaFrontiers in immunology · 2023Article
- A proposed unified interphase nucleus chromosome structure: Preliminary preponderance of evidence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A proposed unified mitotic chromosome architecture.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales.Nature methods · 2022Article
- P2X4 and P2X7 are essential players in basal T cell activity and CaScience advances · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Four-dimensional fluorescence microscopy--which records 3D image information as a function of time--provides an unbiased way of tracking dynamic behavior of subcellular components in living samples and capturing key events in complex macromolecular processes. Unfortunately, the combination of phototoxicity and photobleaching can severely limit the density or duration of sampling, thereby limiting the biological information that can be obtained. Although widefield microscopy provides a very light-efficient way of imaging, obtaining high-quality reconstructions requires deconvolution to remove optical aberrations. Unfortunately, most deconvolution methods perform very poorly at low signal-to-noise ratios, thereby requiring moderate photon doses to obtain acceptable resolution. We present a unique deconvolution method that combines an entropy-based regularization function with kernels that can exploit general spatial characteristics of the fluorescence image to push the required dose to extreme low levels, resulting in an enabling technology for high-resolution in vivo biological imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.