ArticleNucleic acids research2014
Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Chromatin accessibility profile and the role of PeAtf1 transcription factor in the postharvest pathogenHorticulture research · 2025Article
- Psoralen Crosslinking-Chromatin Endogenous Cleavage Assay to Examine Histone DNA Interactions of Active and Inactive rRNA Genes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency.Nucleic acids research · 2023Article
- Review
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Single-Molecule Techniques to Study Chromatin.Frontiers in cell and developmental biology · 2021Review
- Purification and enrichment of specific chromatin loci.Nature methods · 2020Review
- Proximity Labeling Techniques to Study Chromatin.Frontiers in genetics · 2020Review
- Decoding the chromatin proteome of a single genomic locus by DNA sequencing.PLoS biology · 2018Article
- Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin.Scientific reports · 2017Article
- Chromatin potentiates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter.Nature communications · 2015Article
- Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin is the template for replication and transcription in the eukaryotic nucleus, which needs to be defined in composition and structure before these processes can be fully understood. We report an isolation protocol for the targeted purification of specific genomic regions in their native chromatin context from Saccharomyces cerevisiae. Subdomains of the multicopy ribosomal DNA locus containing transcription units of RNA polymerases I, II or III or an autonomous replication sequence were independently purified in sufficient amounts and purity to analyze protein composition and histone modifications by mass spectrometry. We present and discuss the proteomic data sets obtained for chromatin in different functional states. The native chromatin was further amenable to electron microscopy analysis yielding information about nucleosome occupancy and positioning at the single-molecule level. We also provide evidence that chromatin from virtually every single copy genomic locus of interest can be purified and analyzed by this technique.
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