ArticleStem cells (Dayton, Ohio)2014
Membrane biophysics define neuron and astrocyte progenitors in the neural lineage.
Article in Stem cells (Dayton, Ohio), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Microfluidics for cell therapy and manufacturing in oncology and regenerative medicine.Lab on a chip · 2026Review
- Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation.Bioengineering & translational medicine · 2026Article
- Advances in microfluidic platforms for tumor cell phenotyping: from bench to bedside.Lab on a chip · 2025Review
- Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.Stem cell reports · 2023Article
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- Label-free enrichment of fate-biased human neural stem and progenitor cells.Biosensors & bioelectronics · 2020Article
- Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films.ACS biomaterials science & engineering · 2020Article
- High-throughput continuous dielectrophoretic separation of neural stem cells.Biomicrofluidics · 2019Article
- Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis.The Analyst · 2019Article
- Myosin-II mediated traction forces evoke localized Piezo1-dependent CaCommunications biology · 2019Article
- Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells.Stem cell reports · 2018Article
- It's Electric: When Technology Gives a Boost to Stem Cell Science.Current stem cell reports · 2018Article
- Separation of neural stem cells by whole cell membrane capacitance using dielectrophoresis.Methods (San Diego, Calif.) · 2018Review
- Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting.Journal of the Royal Society, Interface · 2017Article
- Dielectrophoresis for Biomedical Sciences Applications: A Review.Sensors (Basel, Switzerland) · 2017Review
- Heat pulse excitability of vestibular hair cells and afferent neurons.Journal of neurophysiology · 2016Article
- Continuous-flow sorting of stem cells and differentiation products based on dielectrophoresis.Lab on a chip · 2015Article
- Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner.Scientific reports · 2015Article
- Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput.Biomicrofluidics · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Neural stem and progenitor cells (NSPCs) are heterogeneous populations of self-renewing stem cells and more committed progenitors that differentiate into neurons, astrocytes, and oligodendrocytes. Accurately identifying and characterizing the different progenitor cells in this lineage has continued to be a challenge for the field. We found previously that populations of NSPCs with more neurogenic progenitors (NPs) can be distinguished from those with more astrogenic progenitors (APs) by their inherent biophysical properties, specifically the electrophysiological property of whole cell membrane capacitance, which we characterized with dielectrophoresis (DEP). Here, we hypothesize that inherent electrophysiological properties are sufficient to define NPs and APs and test this by determining whether isolation of cells solely by these properties specifically separates NPs and APs. We found NPs and APs are enriched in distinct fractions after separation by electrophysiological properties using DEP. A single round of DEP isolation provided greater NP enrichment than sorting with PSA-NCAM, which is considered an NP marker. Additionally, cell surface N-linked glycosylation was found to significantly affect cell fate-specific electrophysiological properties, providing a molecular basis for the cell membrane characteristics. Inherent plasma membrane biophysical properties are thus sufficient to define progenitor cells of differing fate potential in the neural lineage, can be used to specifically isolate these cells, and are linked to patterns of glycosylation on the cell surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.