ArticleOptics express2013
4Pi fluorescence detection and 3D particle localization with a single objective.
Article in Optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed.
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution.Light, science & applications · 2021Article
- mmSTORM: Multimodal localization based super-resolution microscopy.Scientific reports · 2019Article
- Revealing Nanoscale Morphology of the Primary Cilium Using Super-Resolution Fluorescence Microscopy.Biophysical journal · 2019Article
- Article
- Interferometric rotating point spread function.Scientific reports · 2017Article
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Optical tracking of nanoscale particles in microscale environments.Applied physics reviews · 2016Article
- Single-photon-multi-layer-interference lithography for high-aspect-ratio and three-dimensional SU-8 micro-/nanostructures.Scientific reports · 2016Article
- Mirror-enhanced super-resolution microscopy.Light, science & applicationsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) single-molecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance difficulty. To address these issues, we have realized 4Pi fluorescence detection by sandwiching the sample between the objective and a mirror, and create interference of direct incidence and mirror-reflected signal at the camera with a spatial light modulator. Multifocal imaging using this single-objective mirror interference scheme offers improvement in the axial localization similar to the traditional 4Pi method. We have also devised several PSF engineering schemes to enable 3D localization with a single emitter image, offering better axial precision than normal single-objective localization methods such as astigmatic imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.