ArticlePlant molecular biology2014
Identification of a novel type of WRKY transcription factor binding site in elicitor-responsive cis-sequences from Arabidopsis thaliana.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 74 citations in OpenAlex.
- Transcriptional Regulation of the Novel Theacrine Synthase Gene CsTcS2 by the CsTINY-CsWRKY33 Module Underpins Theacrine Biosynthesis in Camellia sinensis.Plant biotechnology journal · 2026Article
- Prefoldin 3 promotes the turnover of WRKY70 and FHY3 to modulate UV-B-induced hypocotyl growth inhibition in Arabidopsis thaliana.Plant communications · 2026Article
- Unravelling the role of WRKY transcription factors in leaf senescence: Genetic and molecular insights.Journal of advanced research · 2025Review
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- Genome-wide analysis of WRKY transcription factors involved in abiotic stress inFrontiers in plant science · 2025Article
- Transcriptome analysis reveals role of transcription factor WRKY70 in early N-hydroxy-pipecolic acid signaling.Plant physiology · 2024Article
- Overexpression of a pearl millet WRKY transcription factor gene, PgWRKY74, in Arabidopsis retards shoot growth under dehydration and salinity-stressed conditions.Biotechnology letters · 2024Article
- Expression of the Arabidopsis redox-related LEA protein, SAG21 is regulated by ERF, NAC and WRKY transcription factors.Scientific reports · 2024Article
- Coordination between two cis-elements of WRKY33, bound by the same transcription factor, confers humid adaption in Arabidopsis thaliana.Plant molecular biology · 2024Article
- OsWRKY26 negatively regulates bacterial blight resistance by suppressingFrontiers in plant science · 2024Article
- Dioscorea composita WRKY5 positively regulates AtSOD1 and AtABF2 to enhance drought and salt tolerances.Plant cell reports · 2023Article
- OsWRKY28 positively regulates salinity tolerance by directly activating OsDREB1B expression in rice.Plant cell reports · 2023Article
- Ethylene enhances resistance to cucumber green mottle mosaic virus via theFrontiers in plant science · 2023Article
- Multifaceted roles of WRKY transcription factors in abiotic stress and flavonoid biosynthesis.Frontiers in plant science · 2023Review
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- Genomic distribution and context dependent functionality of novel WRKY transcription factor binding sites.BMC genomics · 2022Article
- A Comprehensive Study of the WRKY Transcription Factor Family in Strawberry.Plants (Basel, Switzerland) · 2022Article
- Allelic shift in cis-elements of the transcription factorScience advances · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a combination of bioinformatics and synthetic promoters, novel elicitor-responsive cis-sequences were discovered in promoters of pathogen-upregulated genes from Arabidopsis thaliana. One group of functional sequences contains the conserved core sequence GACTTTT. This core sequence and adjacent nucleotides are essential for elicitor-responsive gene expression in a parsley protoplast system. By yeast one-hybrid screening, WRKY70 was selected with a cis-sequence harbouring the core sequence GACTTTT but no known WRKY binding site (W-box). Transactivation experiments, mutation analyses, and electrophoretic mobility shift assays demonstrate that the sequence CGACTTTT is the binding site for WRKY70 in the investigated cis-sequence and is required for WRKY70-activated gene expression. Using several cis-sequences in transactivation experiments and binding studies, the CGACTTTT sequence can be extended to propose YGACTTTT as WRKY70 binding site. This binding site, designated WT-box, is enriched in promoters of genes upregulated in a WRKY70 overexpressing line. Interestingly, functional WRKY70 binding sites are present in the promoter of WRKY30, supporting recent evidence that both factors play a role in the same regulatory network.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.