ArticleThe Plant cell2013
In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed, 173 citations in OpenAlex.
- Root developmental zonation is independent of cell wall pH.Nature plants · 2026Article
- TOP1α-mediated stabilization of CYP82C4 G-quadruplex DNA represses lateral root primordium initiation in Arabidopsis.Nature plants · 2026Article
- Developing downstream processes for the purification of recombinant proteins and small molecules from Nicotiana benthamiana biomass.Plant biotechnology journal · 2026Review
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Expression and characterization of an endo-β-1,6-galactanase from Arabidopsis thaliana.The Biochemical journal · 2025Article
- Delay-facilitated self-assembly in compartmentalized systems.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Gamma-selinene synthase catalyzes the first step of dihydroagarofuran sesquiterpene alkaloid biosynthesis in Tripterygium.Science China. Life sciences · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Cellular pH homeostasis shapes root system architecture by modulating auxin-mediated developmental responses.Plant physiology · 2025Article
- Reverse prenylation in plants by non-canonical aromatic prenyltransferases.The Plant journal : for cell and molecular biology · 2025Article
- The photosensitive endoplasmic reticulum-chloroplast contact site.Journal of microscopy · 2025Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pests.Plant biotechnology journal · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Oligogalactolipid production during cold challenge is conserved in early diverging lineages.Journal of experimental botany · 2023Article
- Article
- Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway.International journal of molecular sciences · 2023Article
- A viral protein disrupts vacuolar acidification to facilitate virus infection in plants.The EMBO journal · 2022Article
- Cell-Type-Specific Length and Cytosolic pH Response of Superficial Cells ofPlants (Basel, Switzerland) · 2022Article
- Biosensing on acid: fluorescent protein probes for low pH environments.Journal of experimental botany · 2022Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pH homeostasis of endomembranes is essential for cellular functions. In order to provide direct pH measurements in the endomembrane system lumen, we targeted genetically encoded ratiometric pH sensors to the cytosol, the endoplasmic reticulum, and the trans-Golgi, or the compartments labeled by the vacuolar sorting receptor (VSR), which includes the trans-Golgi network and prevacuoles. Using noninvasive live-cell imaging to measure pH, we show that a gradual acidification from the endoplasmic reticulum to the lytic vacuole exists, in both tobacco (Nicotiana tabacum) epidermal (ΔpH -1.5) and Arabidopsis thaliana root cells (ΔpH -2.1). The average pH in VSR compartments was intermediate between that of the trans-Golgi and the vacuole. Combining pH measurements with in vivo colocalization experiments, we found that the trans-Golgi network had an acidic pH of 6.1, while the prevacuole and late prevacuole were both more alkaline, with pH of 6.6 and 7.1, respectively. We also showed that endosomal pH, and subsequently vacuolar trafficking of soluble proteins, requires both vacuolar-type H(+) ATPase-dependent acidification as well as proton efflux mediated at least by the activity of endosomal sodium/proton NHX-type antiporters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.