ArticleMolecular systems biology2013
A competitive protein interaction network buffers Oct4-mediated differentiation to promote pluripotency in embryonic stem cells.
Article in Molecular systems biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells.International journal of molecular sciences · 2023Pooled it
- Unveiling gene perturbation effects through gene regulatory networks inference from single-cell transcriptomic data.PLoS computational biology · 2026Article
- Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.PloS one · 2026Article
- Knowledge-guided artificial intelligence technologies for decoding complex multiomics interactions in cells.Clinical and experimental pediatrics · 2022Article
- AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).International journal of molecular sciences · 2021Article
- Auxin-degron system identifies immediate mechanisms of OCT4.Stem cell reports · 2021Article
- Pleiotropic roles of tankyrase/PARP proteins in the establishment and maintenance of human naïve pluripotency.Experimental cell research · 2020Review
- Novel Approaches to Profile Functional Long Noncoding RNAs Associated with Stem Cell Pluripotency.Current genomics · 2020Review
- Nessys: A new set of tools for the automated detection of nuclei within intact tissues and dense 3D cultures.PLoS biology · 2019Article
- Review
- A common molecular logic determines embryonic stem cell self-renewal and reprogramming.The EMBO journal · 2019Article
- No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts.PloS one · 2019Article
- A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells.Nature communications · 2018Article
- Construction of Discrete Model of Human Pluripotency in Predicting Lineage-Specific Outcomes and Targeted Knockdowns of Essential Genes.Scientific reports · 2018Article
- Network Features and Dynamical Landscape of Naive and Primed Pluripotency.Biophysical journal · 2018Article
- Capturing Human Naïve Pluripotency in the Embryo and in the Dish.Stem cells and development · 2017Review
- Article
- Nanog Dynamics in Mouse Embryonic Stem Cells: Results from Systems Biology Approaches.Stem cells international · 2017Review
- Dissecting mechanisms of mouse embryonic stem cells heterogeneity through a model-based analysis of transcription factor dynamics.Journal of the Royal Society, Interface · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Pluripotency in embryonic stem cells is maintained through the activity of a small set of transcription factors centred around Oct4 and Nanog, which control the expression of 'self-renewal' and 'differentiation' genes. Here, we combine single-cell quantitative immunofluorescence microscopy and gene expression analysis, together with theoretical modelling, to investigate how the activity of those factors is regulated. We uncover a key role for post-translational regulation in the maintenance of pluripotency, which complements the well-established transcriptional regulatory layer. Specifically, we find that the activity of a network of protein complexes involving Nanog, Oct4, Tcf3, and β-catenin suffices to account for the behavior of ES cells under different conditions. Our results suggest that the function of the network is to buffer the transcriptional activity of Oct4, which appears to be the main determinant to exit pluripotency. The protein network explains the mechanisms underlying the gain and loss of function in different mutants, and brings us closer to a full understanding of the molecular basis of pluripotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.