ArticleNutrition (Burbank, Los Angeles County, Calif.)
L-Threonine induces heat shock protein expression and decreases apoptosis in heat-stressed intestinal epithelial cells.
Article in Nutrition (Burbank, Los Angeles County, Calif.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Effect of rumen-protected L-tryptophan on productivity and metabolic responses in dry Holstein cows under heat stress conditions.Scientific reports · 2026Article
- Combined Analysis of Transcriptome and Metabolome Reveals the Heat Stress Resistance of Dongxiang Wild Rice at Seedling Stage.Plants (Basel, Switzerland) · 2025Article
- Increasing concentrations of dietary threonine, tryptophan, and glycine improve growth performance and intestinal health with decreasing stress responses in broiler chickens raised under multiple stress conditions.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Exploring the Heat-Responsive miRNAs and their Target Gene Regulation in Ruditapes philippinarum Under Acute Heat Stress.Marine biotechnology (New York, N.Y.) · 2024Article
- The biphasic role of Hspb1 on ferroptotic cell death in Parkinson's disease.Theranostics · 2024Article
- Alleviative Effect of Threonine on Cadmium-Induced Liver Injury in Mice.Biological trace element research · 2023Article
- Effects of functional nutrients on chicken intestinal epithelial cells induced with oxidative stress.Journal of animal science and technology · 2023Article
- Article
- An acute increase in water temperature can increase free amino acid concentrations in the blood, brain, liver, and muscle in goldfish (Carassius auratus).Fish physiology and biochemistry · 2019Article
- Dietary Nutrients and Bioactive Substances Modulate Heat Shock Protein (HSP) Expression: A Review.Nutrients · 2018Review
- Review
- Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota.Nutrition reviews · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesOsmotically acting amino acids can be cytoprotective following injury. As threonine (THR) induces osmotic cell swelling, our aim was to investigate the potential for THR to induce cellular protection in intestinal epithelial cells and evaluate possible mechanisms of protection.
methodsCells treated with a range of THR doses were evaluated following heat stress (HS) injury. Alpha-aminoisobutyric acid (AIB), a non-metabolizable amino acid analog, was used as an osmotic control. MTS assays were used to assess cell survival. Heat shock protein (HSP) expression and cleaved caspase-3 (CC3) were evaluated via Western blot. Cell morphology and cell size were analyzed via microscopy.
resultFollowing HS, THR treatment increased cell viability in a dose dependent manner vs. non-THR treated cells (CT). The non-metabolized amino acid analogue, AIB, also increased cell survival in heat-stressed cells versus HS controls. HSP70 and HSP25 expression increased with THR and AIB treatment versus HS controls. THR also increased HSP25 in non-stressed cells. Microscopic evaluation revealed both THR and AIB preserved the structural integrity of the actin cytoskeleton in heat-stressed cells versus HS controls. THR, but not AIB, enhanced nuclear translocation of HSP25 during HS. This nuclear translocation was associated with a 60% decrease in apoptosis in heat-stressed cells with THR. No antiapoptotic effect was observed with AIB.
conclusionsThis is the first demonstration that THR increases HSP70 and HSP 25 and protects cells from HS. THR's mechanism of protection may involve cytoskeletal stabilization, HSP up-regulation and nuclear translocation, and decreased apoptosis. THR's protection appears to involve both cell-swelling-dependent and -independent processes.
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