ArticleMolecular plant pathology2014
Analysis of Tomato spotted wilt virus NSs protein indicates the importance of the N-terminal domain for avirulence and RNA silencing suppression.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 89 citations in OpenAlex.
- Emergence and molecular characterization of a resistance-breaking tomato spotted wilt virus isolate on bell pepper plants harboring the Tsw resistance gene in Japan.Archives of virology · 2026Article
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- Wild-type and resistance-breaking strains of tomato spotted wilt virus differentially upregulate the immunosuppressive epoxyoctadecamonoenoic acid biosynthesis of its insect vector,The Journal of general virology · 2025Article
- Molecular genetic analyses of the N, NSm and NSs genes of a local population ofThe Journal of general virology · 2025Article
- Identification of novel sources of resistance to squash leaf curl China virus (SLCCNV) and cucurbit chlorotic yellows virus (CCYV) and inheritance analysis of SLCCNV resistance in pumpkin (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
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- P4 protein of an Indian isolate of rice tungro bacilliform virus modulates gene silencing.Virus genes · 2024Article
- Signaling and Resistosome Formation in Plant Innate Immunity to Viruses: Is There a Common Mechanism of Antiviral Resistance Conserved across Kingdoms?International journal of molecular sciences · 2023Review
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- Small RNAs Participate in Plant-Virus Interaction and Their Application in Plant Viral Defense.International journal of molecular sciences · 2022Review
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- Influence of virus-host interactions on plant response to abiotic stress.Plant cell reports · 2021Review
- Impact of Host Resistance to Tomato Spotted Wilt Orthotospovirus in Peanut Cultivars on Virus Population Genetics and Thrips Fitness.Pathogens (Basel, Switzerland) · 2021Article
- The Bunyavirales: The Plant-Infecting Counterparts.Viruses · 2021Review
- Review
- Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors.Pathogens (Basel, Switzerland) · 2021Review
- Identification and Characterization of Plant-Interacting Targets of Tomato Spotted Wilt Virus Silencing Suppressor.Pathogens (Basel, Switzerland) · 2021Article
- Estimation of the functions of viral RNA silencing suppressors by apple latent spherical virus vector.Virus genes · 2020Article
- Rescue of tomato spotted wilt virus entirely from complementary DNA clones.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, Tomato spotted wilt virus (TSWV) nonstructural protein NSs has been identified unambiguously as an avirulence (Avr) determinant for Tomato spotted wilt (Tsw)-based resistance. The observation that NSs from two natural resistance-breaking isolates had lost RNA silencing suppressor (RSS) activity and Avr suggested a link between the two functions. To test this, a large set of NSs mutants was generated by alanine substitutions in NSs from resistance-inducing wild-type strains (NSs(RI) ), amino acid reversions in NSs from resistance-breaking strains (NSs(RB)), domain deletions and swapping. Testing these mutants for their ability to suppress green fluorescent protein (GFP) silencing and to trigger a Tsw-mediated hypersensitive response (HR) revealed that the two functions can be separated. Changes in the N-terminal domain were found to be detrimental for both activities and indicated the importance of this domain, additionally supported by domain swapping between NSs(RI) and NSs(RB). Swapping domains between the closely related Tospovirus Groundnut ringspot virus (GRSV) NSs and TSWV NSs(RI) showed that Avr functionality could not simply be transferred between species. Although deletion of the C-terminal domain rendered NSs completely dysfunctional, only a few single-amino-acid mutations in the C-terminus affected both functions. Mutation of a GW/WG motif (position 17/18) rendered NSs completely dysfunctional for RSS and Avr activity, and indicated a putative interaction between NSs and Argonaute 1 (AGO1), and its importance in TSWV virulence and viral counter defence against RNA interference.
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