ArticleAnalytical chemistry2013
Detection of extracellular RNAs in cancer and viral infection via tethered cationic lipoplex nanoparticles containing molecular beacons.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- A novel nanosensor for in situ detection of EV-miR-107 enables noninvasive tuberculosis diagnosis and treatment monitoring.Journal of nanobiotechnology · 2026Article
- A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer.Cancer biology & therapy · 2025Article
- Extracellular Vesicular Delta-Like Ligand 3 and Subtype Transcription Factors for Small Cell Lung Cancer Diagnosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content.Advanced healthcare materials · 2025Article
- Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Engineering a tunable micropattern-array assay to sort single extracellular vesicles and particles to detect RNA and protein in situ.Journal of extracellular vesicles · 2023Article
- Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis.ACS nano · 2023Article
- Loading of "cocktail siRNAs" into extracellular vesicles via TAT-DRBD peptide for the treatment of castration-resistant prostate cancer.Cancer biology & therapy · 2022Article
- Recent advances in nanotechnology-enabled biosensors for detection of exosomes as new cancer liquid biopsy.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- An immunogold single extracellular vesicular RNA and protein (Journal of extracellular vesicles · 2022Article
- Multiplexed Profiling of Extracellular Vesicles for Biomarker Development.Nano-micro letters · 2021Review
- Microfluidic-Chip-Integrated Biosensors for Lung Disease Models.Biosensors · 2021Review
- Nanotechnology in emerging liquid biopsy applications.Nano convergence · 2021Review
- Ultrafast Detection of Exosomal RNAsACS applied nano materials · 2021Article
- Elevated expression of exosomal microRNA-21 as a potential biomarker for the early diagnosis of pancreatic cancer using a tethered cationic lipoplex nanoparticle biochip.Oncology letters · 2020Article
- Non-invasive Detection of Exosomal MicroRNAs via Tethered Cationic Lipoplex Nanoparticles (tCLN) Biochip for Lung Cancer Early Detection.Frontiers in genetics · 2020Article
- Glycerol kinase 5 confers gefitinib resistance through SREBP1/SCD1 signaling pathway.Journal of experimental & clinical cancer research : CR · 2019Article
- A Review of Exosomes and their Role in The Tumor Microenvironment and Host-Tumor "Macroenvironment".Journal of immunological sciences · 2019Article
- An Immuno-Biochip Selectively Captures Tumor-Derived Exosomes and Detects Exosomal RNAs for Cancer Diagnosis.ACS applied materials & interfaces · 2018Article
- Ultrasensitive quantification of tumor mRNAs in extracellular vesicles with an integrated microfluidic digital analysis chip.Lab on a chip · 2018Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Noninvasive early detection methods have the potential to reduce mortality rates of both cancer and infectious diseases. Here, we present a novel assay by which tethered cationic lipoplex nanoparticles containing molecular beacons (MBs) can capture cancer cell-derived exosomes or viruses and identify encapsulated RNAs in a single step. A series of ultracentrifugation and Exoquick isolation kit were first used to isolate exosomes from the cell culture medium and human serum, respectively. Cationic lipoplex nanoparticles linked onto the surface of a thin glass plate capture negatively charged viruses or cell-secreted exosomes by electrostatic interactions to form larger nanoscale complexes. Lipoplex/virus or lipoplex/exosome fusion leads to the mixing of viral/exosomal RNAs and MBs within the lipoplexes. After the target RNAs specially bind to the MBs, exosomes enriched in target RNAs are readily identified by the fluorescence signals of MBs. The in situ detection of target extracellular RNAs without diluting the samples leads to high detection sensitivity not achievable by existing methods, e.g., quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Here we demonstrate this concept using lentivirus and serum from lung cancer patients.
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