ArticleProceedings of the National Academy of Sciences of the United States of America2013
Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Dynamics in Cre-loxP site-specific recombination.Current opinion in structural biology · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Oriented Soft DNA Curtains for Single-Molecule Imaging.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Functional Analysis of theAntibiotics (Basel, Switzerland) · 2020Article
- The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies.The Journal of biological chemistry · 2019Article
- Tethered multifluorophore motion reveals equilibrium transition kinetics of single DNA double helices.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- Xer Site Specific Recombination: Double and Single Recombinase Systems.Frontiers in microbiology · 2017Review
- Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending.eLife · 2016Article
- Activation of Xer-recombination at dif: structural basis of the FtsKγ-XerD interaction.Scientific reports · 2016Article
- How Xer-exploiting mobile elements overcome cellular control.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Assembly, translocation, and activation of XerCD-dif recombination by FtsK translocase analyzed in real-time by FRET and two-color tethered fluorophore motion.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- XerD-mediated FtsK-independent integration of TLCϕ into the Vibrio cholerae genome.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Tethered fluorophore motion: studying large DNA conformational changes by single-fluorophore imaging.Biophysical journal · 2014Article
- Single-molecule imaging of FtsK translocation reveals mechanistic features of protein-protein collisions on DNA.Molecular cell · 2014Article
- Studying DNA-protein interactions with single-molecule Förster resonance energy transfer.Protoplasma · 2014Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Three single-molecule techniques have been used simultaneously and in tandem to track the formation in vitro of single XerCD-dif recombination complexes. We observed the arrival of the FtsK translocase at individual preformed synaptic complexes and demonstrated the conformational change that occurs during their activation. We then followed the reaction intermediate transitions as Holliday junctions formed through catalysis by XerD, isomerized, and were converted by XerC to reaction products, which then dissociated. These observations, along with the calculated intermediate lifetimes, inform the reaction mechanism, which plays a key role in chromosome unlinking in most bacteria with circular chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.