ArticleProceedings of the National Academy of Sciences of the United States of America2013
DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Structural interplay between DNA-shape protein recognition and supercoiling: The case of IHF.Computational and structural biotechnology journal · 2022Article
- Single-molecule insights into torsion and roadblocks in bacterial transcript elongation.Transcription · 2021Article
- Protein-mediated looping of DNA under tension requires supercoiling.Nucleic acids research · 2018Article
- The Developmental Switch in Bacteriophage λ: A Critical Role of the Cro Protein.Journal of molecular biology · 2018Article
- Interference between Triplex and Protein Binding to Distal Sites on Supercoiled DNA.Biophysical journal · 2017Article
- Refining particle positions using circular symmetry.PloS one · 2017Article
- Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics.Methods in enzymology · 2017Article
- The interplay between DNA topology and accessory factors in site-specific recombination in bacteria and their bacteriophages.Science progress · 2016Review
- DNA supercoiling during transcription.Biophysical reviews · 2016Article
- Protein/DNA interactions in complex DNA topologies: expect the unexpected.Biophysical reviews · 2016Review
- Supercoiling Effects on Short-Range DNA Looping in E. coli.PloS one · 2016Article
- Protein/DNA interactions in complex DNA topologies: expect the unexpected.Biophysical reviews · 2016Review
- Cooperativity leads to temporally-correlated fluctuations in the bacteriophage lambda genetic switch.Frontiers in plant science · 2015Article
- DNA supercoiling: a regulatory signal for the λ repressor.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Quantitation of interactions between two DNA loops demonstrates loop domain insulation in E. coli cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Tethered particle analysis of supercoiled circular DNA using peptide nucleic acid handles.Nature protocols · 2014Article
- Variola type IB DNA topoisomerase: DNA binding and supercoil unwinding using engineered DNA minicircles.Biochemistry · 2014Article
- Effect of supercoiling on the λ switch.Bacteriophage · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.