ArticlePlant molecular biology2014
ACA12 is a deregulated isoform of plasma membrane Ca²⁺-ATPase of Arabidopsis thaliana.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- A systems biology framework integrating cross-species transcriptomics and PPI networks for Xylella fastidiosa resistance gene identification.BMC plant biology · 2025Article
- Evolutionarily conserved BON1 regulates the basal cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- Hypothesis paper: the development of a regulatory layer in P2B autoinhibited CaPlant signaling & behavior · 2023Article
- Resting cytosol Ca2+ level maintained by Ca2+ pumps affects environmental responses in Arabidopsis.Plant physiology · 2023Article
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Article
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Proton and calcium pumping P-type ATPases and their regulation of plant responses to the environment.Plant physiology · 2021Review
- Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots.Molecular systems biology · 2021Article
- Arabidopsis Ca2+-ATPases 1, 2, and 7 in the endoplasmic reticulum contribute to growth and pollen fitness.Plant physiology · 2021Article
- Overlapping and differential roles of plasma membrane calcium ATPases in Arabidopsis growth and environmental responses.Journal of experimental botany · 2018Article
- Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis).Scientific reports · 2017Article
- Vacuolar Chloride Fluxes Impact Ion Content and Distribution during Early Salinity Stress.Plant physiology · 2016Article
- Functional characterization of a Glycine soja Ca(2+)ATPase in salt-alkaline stress responses.Plant molecular biology · 2016Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Plant auto-inhibited Ca²⁺-ATPases (ACA) are crucial in defining the shape of calcium transients and therefore in eliciting plant responses to various stimuli. Arabidopsis thaliana genome encodes ten ACA isoforms that can be divided into four clusters based on gene structure and sequence homology. While isoforms from clusters 1, 2 and 4 have been characterized, virtually nothing is known about members of cluster 3 (ACA12 and ACA13). Here we show that a GFP-tagged ACA12 localizes at the plasma membrane and that expression of ACA12 rescues the phenotype of partial male sterility of a null mutant of the plasma membrane isoform ACA9, thus providing genetic evidence that ACA12 is a functional plasma membrane-resident Ca²⁺-ATPase. By ACA12 expression in yeast and purification by CaM-affinity chromatography, we show that, unlike other ACAs, the activity of ACA12 is not stimulated by CaM. Moreover, full length ACA12 is able to rescue a yeast mutant deficient in calcium pumps. Analysis of single point ACA12 mutants suggests that ACA12 loss of auto-inhibition can be ascribed to the lack of two acidic residues--highly conserved in other ACA isoforms--localized at the cytoplasmic edge of the second and third transmembrane segments. Together, these results support a model in which the calcium pump activity of ACA12 is primarily regulated by increasing or decreasing mRNA expression and/or protein translation and degradation.
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