ArticleMolecular and cellular biology2013
Intra- and intermolecular regulatory interactions in Upf1, the RNA helicase central to nonsense-mediated mRNA decay in yeast.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Anti-Prion Systems in Saccharomyces cerevisiae.Journal of neurochemistry · 2025Review
- Accurate and sensitive interactome profiling using a quantitative protein-fragment complementation assay.Cell reports methods · 2024Article
- Rapid functional activation of horizontally transferred eukaryotic intron-containing genes in the bacterial recipient.Nucleic acids research · 2024Article
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- Principles, mechanisms, and biological implications of translation termination-reinitiation.RNA (New York, N.Y.) · 2023Article
- Ribosome-bound Upf1 forms distinct 80S complexes and conducts mRNA surveillance.RNA (New York, N.Y.) · 2022Article
- Review
- UPF1-Mediated RNA Decay-Danse Macabre in a Cloud.Biomolecules · 2020Review
- Selective profiling of ribosomes associated with yeast Upf proteins.Methods (San Diego, Calif.) · 2019Article
- Anti-prion systems in yeast.The Journal of biological chemistry · 2019Review
- Nonsense-mediated mRNA decay factors cure most [PSI+] prion variants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- ATP hydrolysis by UPF1 is required for efficient translation termination at premature stop codons.Nature communications · 2016Article
- Chemical-Induced Read-Through at Premature Termination Codons Determined by a Rapid Dual-Fluorescence System Based on S. cerevisiae.PloS one · 2016Article
- Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain.RNA (New York, N.Y.) · 2015Article
- Review
- Nonsense suppression by near-cognate tRNAs employs alternative base pairing at codon positions 1 and 3.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.Annual review of genetics · 2015Review
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
RNA helicases are involved in almost every aspect of RNA metabolism, yet very little is known about the regulation of this class of enzymes. In Saccharomyces cerevisiae, the stability and translational fidelity of nonsense-containing mRNAs are controlled by the group I RNA helicase Upf1 and the proteins it interacts with, Upf2 and Upf3. Combining the yeast two-hybrid system with genetic analysis, we show here that the cysteine- and histidine-rich (CH) domain and the RNA helicase domain of yeast Upf1 can engage in two new types of molecular interactions: an intramolecular interaction between these two domains and self-association of each of these domains. Multiple observations indicate that these molecular interactions are crucial for Upf1 regulation. First, coexpression of the CH domain and the RNA helicase domain in trans can reconstitute Upf1 function in both promoting nonsense-mediated mRNA decay (NMD) and preventing nonsense suppression. Second, mutations that disrupt Upf1 intramolecular interaction cause loss of Upf1 function. These mutations weaken Upf2 interaction and, surprisingly, promote Upf1 self-association. Third, the genetic defects resulting from deficiency in Upf1 intramolecular interaction or RNA binding are suppressed by expression of Upf2. Collectively, these data reveal a set of sequential molecular interactions and their roles in regulating Upf1 function during activation of NMD and suggest that cis intramolecular interaction and trans self-association may be general mechanisms for regulation of RNA helicase functions.
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