ArticleThe Journal of physiology2013
Identification of unique release kinetics of serotonin from guinea-pig and human enterochromaffin cells.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 78 citations in OpenAlex.
- Plasma levels of adrenaline, noradrenaline, dopamine, serotonin, and cortisol in sarcopenia: comparison with non-sarcopenic individuals and the impact of resistance exercise and nutrition interventions.Journal of the International Society of Sports Nutrition · 2025Trial
- Metformin-induced glucagon-like peptide-1 secretion contributes to the actions of metformin in type 2 diabetes.JCI insight · 2018Trial
- Insights into small-molecule neurotransmitter levels and distribution during tissue regeneration in an ear pinna model in mice.Scientific reports · 2026Article
- Protease-Activated Receptor 2 Activation Provokes an Increase in Intracellular Calcium and Serotonin Secretion in a Human Enteroendocrine Cell Line.Cell biochemistry and function · 2025Article
- Cold and hot consumption and health outcomes among US Asian and White populations.The British journal of nutrition · 2025Article
- Effects of selective serotonin reuptake inhibitors on the placenta†.Biology of reproduction · 2025Review
- Serotonin activates dermal papilla cells and promotes hair growth.Scientific reports · 2025Article
- Population imaging of enterochromaffin cell activity reveals regulation by somatostatin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enteric Nervous System: Identification of a Novel Neuronal Sensory Network in the Duodenal Epithelium.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Nanoformulation improves antitumor efficacy of MAOI immune checkpoint blockade therapy without causing aggression-related side effects.Frontiers in pharmacology · 2022Article
- Recent advances in biofabricated gut models to understand the gut-brain axis in neurological diseases.Frontiers in medical technology · 2022Review
- Potential Roles of Enterochromaffin Cells in Early Life Stress-Induced Irritable Bowel Syndrome.Frontiers in cellular neuroscience · 2022Review
- Chronic Intestinal Pseudo-Obstruction: Is There a Connection with Gut Microbiota?Microorganisms · 2021Review
- A Budding Relationship: Bacterial Extracellular Vesicles in the Microbiota-Gut-Brain Axis.International journal of molecular sciences · 2020Review
- Enteric nervous system: sensory transduction, neural circuits and gastrointestinal motility.Nature reviews. Gastroenterology & hepatology · 2020Review
- Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes.Microsystems & nanoengineering · 2020Article
- Sugar Responses of Human Enterochromaffin Cells Depend on Gut Region, Sex, and Body Mass.Nutrients · 2019Article
- The Influence of the Gut Microbiome on Host Metabolism Through the Regulation of Gut Hormone Release.Frontiers in physiology · 2019Article
- Models and Tools for Studying Enteroendocrine Cells.Endocrinology · 2018Review
- Augmented capacity for peripheral serotonin release in human obesity.International journal of obesity (2005) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major source of serotonin (5-HT) in the body is the enterochromaffin (EC) cells lining the intestinal mucosa of the gastrointestinal tract. Despite the fact that EC cells synthesise ∼95% of total body 5-HT, and that this 5-HT has important paracrine and endocrine roles, no studies have investigated the mechanisms of 5-HT release from single primary EC cells. We have developed a rapid primary culture of guinea-pig and human EC cells, allowing analysis of single EC cell function using electrophysiology, electrochemistry, Ca(2+) imaging, immunocytochemistry and 3D modelling. Ca(2+) enters EC cells upon stimulation and triggers quantal 5-HT release via L-type Ca(2+) channels. Real time amperometric techniques reveal that EC cells release 5-HT at rest and this release increases upon stimulation. Surprisingly for an endocrine cell storing 5-HT in large dense core vesicles (LDCVs), EC cells release 70 times less 5-HT per fusion event than catecholamine released from similarly sized LDCVs in endocrine chromaffin cells, and the vesicle release kinetics instead resembles that observed in mammalian synapses. Furthermore, we measured EC cell density along the gastrointestinal tract to create three-dimensional (3D) simulations of 5-HT diffusion using the minimal number of variables required to understand the physiological relevance of single cell 5-HT release in the whole-tissue milieu. These models indicate that local 5-HT levels are likely to be maintained around the activation threshold for mucosal 5-HT receptors and that this is dependent upon stimulation and location within the gastrointestinal tract. This is the first study demonstrating single cell 5-HT release in primary EC cells. The mode of 5-HT release may represent a unique mode of exocytosis amongst endocrine cells and is functionally relevant to gastrointestinal sensory and motor function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.