ArticleBMC research notes2013
Selection of reference genes for quantitative real-time PCR analysis in chicken embryo fibroblasts infected with avian leukosis virus subgroup J.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
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- Embryonic transcriptome unravels mechanisms and pathways underlying embryonic development with respect to muscle growth, egg production, and plumage formation in native and broiler chickens.Frontiers in genetics · 2022Article
- Investigation of chicken housekeeping genes using next-generation sequencing data.Frontiers in genetics · 2022Article
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- Multi-carbohydrase enzymes improve feed energy in broiler diets containing standard or low crude protein.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Monoglyceride Blend Reduces Mortality, Improves Nutrient Digestibility, and Intestinal Health in Broilers Subjected to Clinical Necrotic Enteritis Challenge.Animals : an open access journal from MDPI · 2021Article
- Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.Frontiers in physiology · 2021Article
- Over-processed meat and bone meal and phytase effects on broilers challenged with subclinical necrotic enteritis: Part 2. Inositol phosphate esters hydrolysis, intestinal permeability, hematology, jejunal gene expression and intestinal morphology.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Article
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- Identification of Reference Genes in Chicken Intraepithelial Lymphocyte Natural Killer Cells Infected with Very-virulent Infectious Bursal Disease Virus.Scientific reports · 2020Article
- Article
- Validation of the Reference Genes for the Gene Expression Studies in Chicken DT40 Cell Line.Genes · 2020Article
- Ginseng Extract Ameliorates the Negative Physiological Effects of Heat Stress by Supporting Heat Shock Response and Improving Intestinal Barrier Integrity: Evidence from Studies with Heat-Stressed Caco-2 Cells,Molecules (Basel, Switzerland) · 2020Article
- Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.Molecular biology reports · 2020Article
- Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection.Scientific reports · 2018Article
- Article
- Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus.Scientific reports · 2017Article
- Downregulation of ALAS1 by nicarbazin treatment underlies the reduced synthesis of protoporphyrin IX in shell gland of laying hens.Scientific reports · 2017Article
- Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin.PloS one · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe selection of stably expressed reference genes is a prerequisite when evaluating gene expression, via real-time PCR, in cells in response to viral infections. The objective of our study was to identify suitable reference genes for mRNA expression analysis in chicken embryonic fibroblasts (CEF) after infection with avian leukosis virus subgroup J (ALV-J).
findingsThe expression levels of 11 potential reference genes in CEF infected with ALV-J were determined by real-time PCR. The expression stability of these genes were analyzed and ranked using the geNorm tool. Analysis indicated that the genes RPL30 (ribosomal protein L30) and SDHA (succinate dehydrogenase complex, subunit A) were the most stably expressed genes in the ALV-J infected CEF.
conclusionsThe RPL30 and SDHA were deemed suitable for use as reference genes for real-time PCR analysis of mRNA gene expression during ALV-J infection, whereas commonly used ACTB and GAPDH are unsuitable to be reference genes.
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