ArticleJournal of the American Chemical Society2013
Optically modulatable blue fluorescent proteins.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Review of FRET biosensing and its application in biomolecular detection.American journal of translational research · 2023Review
- Chromophore reduction plus reversible photobleaching: how the mKate2 "photoconversion" works.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.The journal of physical chemistry. B · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Structure-guided evolution of Green2 toward photostability and quantum yield enhancement by F145Y substitution.Protein science : a publication of the Protein Society · 2020Article
- Facile autofluorescence suppression enabling tracking of single viruses in live cells.The Journal of biological chemistry · 2019Article
- Optically Activated Delayed Fluorescence.The journal of physical chemistry letters · 2017Article
- Improved Fluorescent Protein Contrast and Discrimination by Optically Controlling Dark State Lifetimes.The journal of physical chemistry letters · 2017Article
- Dark State-Modulated Fluorescence Correlation Spectroscopy for Quantitative Signal Recovery.The journal of physical chemistry letters · 2016Article
- Optically Modulated Photoswitchable Fluorescent Proteins Yield Improved Biological Imaging Sensitivity.Journal of the American Chemical Society · 2015Article
- Expanding discriminative dimensions for analysis and imaging.Chemical science · 2015Article
- Tailoring cyanine dark states for improved optically modulated fluorescence recovery.The journal of physical chemistry. B · 2015Article
- Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background.Accounts of chemical research · 2014Review
- Genetic incorporation of histidine derivatives using an engineered pyrrolysyl-tRNA synthetase.ACS chemical biology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Blue fluorescent proteins (BFPs) offer visualization of protein location and behavior, but often suffer from high autofluorescent background and poor signal discrimination. Through dual-laser excitation of bright and photoinduced dark states, mutations to the residues surrounding the BFP chromophore enable long-wavelength optical modulation of BFP emission. Such dark state engineering enables violet-excited blue emission to be increased upon lower energy, green coillumination. Turning this green coillumination on and off at a specific frequency dynamically modulates collected blue fluorescence without generating additional background. Interpreted as transient photoconversion between neutral cis and anionic trans chromophoric forms, mutations tune photoisomerization and ground state tautomerizations to enable long-wavelength depopulation of the millisecond-lived, spectrally shifted dark states. Single mutations to the tyrosine-based blue fluorescent protein T203V/S205V exhibit enhanced modulation depth and varied frequency. Importantly, analogous single point mutations in the nonmodulatable BFP, mKalama1, creates a modulatable variant. Building modulatable BFPs offers opportunities for improved BFP signal discrimination vs background, greatly enhancing their utility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.