ArticleMethods in cell biology2013
Analysis of lipid droplets in cardiac muscle.
Article in Methods in cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Ultrastructure analysis of mitochondria, lipid droplet and sarcoplasmic reticulum apposition in human heart failure.Journal of molecular and cellular cardiology plus · 2025Article
- Lipid exposure leads to metabolic dysfunction in fetal sheep cardiomyocytes.Physiological reports · 2025Article
- Rational Design of Pyrido[3,2-JACS Au · 2024Article
- Physiological response to fetal intravenous lipid emulsion.Clinical science (London, England : 1979) · 2024Article
- Isolation and Lipidomic Profiling of Neuronal Lipid Droplets: Unveiling the Lipid Landscape for insights into Neurodegenerative Disorders.bioRxiv : the preprint server for biology · 2023Article
- Thyroid hormone increases fatty acid use in fetal ovine cardiac myocytes.Physiological reports · 2023Article
- Intrauterine growth restriction elevates circulating acylcarnitines and suppresses fatty acid metabolism genes in the fetal sheep heart.The Journal of physiology · 2022Article
- Hydrogen sulphide reduced the accumulation of lipid droplets in cardiac tissues of db/db mice via Hrd1 S-sulfhydration.Journal of cellular and molecular medicine · 2021Article
- Formation and characterization of lipid droplets of the bovine corpus luteum.Scientific reports · 2020Article
- Two Types of Contact Between Lipid Droplets and Mitochondria.Frontiers in cell and developmental biology · 2020Review
- Quantitative proteomics and single-nucleus transcriptomics of the sinus node elucidates the foundation of cardiac pacemaking.Nature communications · 2019Article
- Physiological and pathological roles of FATP-mediated lipid droplets in Drosophila and mice retina.PLoS genetics · 2018Article
- Lipid Droplet Biogenesis.Annual review of cell and developmental biology · 2017Review
- Microscopy tools for the investigation of intracellular lipid storage and dynamics.Molecular metabolism · 2016Review
- 3D imaging of the early embryonic chicken heart with focused ion beam scanning electron microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2014Article
- Rab proteins implicated in lipid storage and mobilization.Journal of biomedical research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cellular energy homeostasis is a crucial function of oxidative tissues but becomes altered with obesity, a major health problem that is rising unabated and demands attention. Maintaining cardiac lipid homeostasis relies on complex processes and pathways that require concerted actions between lipid droplets (LDs) and mitochondria to prevent intracellular accumulation of bioactive or toxic lipids while providing an efficient supply of lipid for conversion into ATP. While cardiac mitochondria have been extensively studied, cardiac LDs and their role in heart function have not been fully characterized. The cardiac LD compartment is highly dynamic and individual LD is small, making their study challenging. Here, we describe a simple procedure to isolate cardiac LDs that provide sufficient amounts of highly enriched material to allow subsequent protein and lipid biochemical characterization. We also present a detailed protocol to image cardiac LDs by conventional transmission electronic microscopy to provide two-dimensional (2D) analyses of cardiac LDs and mitochondria. Finally, we discuss the potential advantages of dual ion beam and electron beam platform (FIB-SEM) technology to study the cardiac LDs and mitochondria by allowing 3D imaging analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.