ArticleAnalytical chemistry2013
Self-digitization of samples into a high-density microfluidic bottom-well array.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- High-Density Microfluidic Chip with Vertical Structure for Digital PCR.Sensors (Basel, Switzerland) · 2025Article
- A reinforced PDMS mold for hot embossing of cyclic olefin polymer in the fabrication of microfluidic chips.Lab on a chip · 2022Article
- A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis.Biosensors · 2021Review
- Article
- Computational Modelling and Big Data Analysis of Flow and Drug Transport in Microfluidic Systems: A Spheroid-on-a-Chip Study.Frontiers in bioengineering and biotechnology · 2021Article
- A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR.Micromachines · 2020Article
- Enhanced sample filling and discretization in thermoplastic 2D microwell arrays using asymmetric contact angles.Biomicrofluidics · 2020Article
- A microfluidic alternating-pull-push active digitization method for sample-loss-free digital PCR.Lab on a chip · 2019Article
- Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP.Lab on a chip · 2019Article
- Statistical Analysis of Nonuniform Volume Distributions for Droplet-Based Digital PCR Assays.Journal of the American Chemical Society · 2019Article
- SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA).Lab on a chip · 2018Article
- An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood.Biomicrofluidics · 2018Article
- A Self-Digitization Dielectrophoretic (SD-DEP) Chip for High-Efficiency Single-Cell Capture, On-Demand Compartmentalization, and Downstream Nucleic Acid Analysis.Angewandte Chemie (International ed. in English) · 2018Article
- dPCR: A Technology Review.Sensors (Basel, Switzerland) · 2018Review
- Simultaneous detection of multiple HPV DNA via bottom-well microfluidic chip within an infra-red PCR platform.Biomicrofluidics · 2018Article
- Article
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Digital PCR using micropatterned superporous absorbent array chips.The Analyst · 2016Article
- Self-digitization microfluidic chip for absolute quantification of mRNA in single cells.Analytical chemistry · 2014Article
- Microfluidics for single-cell genetic analysis.Lab on a chip · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
This paper describes a sample digitization method that generates tens of thousands of nanoliter-sized droplets in a high-density array in a matter of minutes. We show that the sample digitization depends on both the geometric design of the microfluidic device and the viscoelastic forces between the aqueous sample and a continuous oil phase. Our design avoids sample loss: Samples are split into tens of thousands of discrete volumes with close to 100% efficiency without the need for any expensive valving or pumping systems. We envision this technology will have broad applications that require simple sample digitization within minutes, such as digital polymerase chain reactions and single-cell studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.