ArticlePLoS neglected tropical diseases2013
Pathogenomic inference of virulence-associated genes in Leptospira interrogans.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 62 citations in OpenAlex.
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- Genomic analysis of human-infecting Leptospira borgpetersenii isolates in Sri Lanka: Expanded PF07598 gene family repertoire and less genome reduction than bovine isolates.PLoS neglected tropical diseases · 2026Article
- In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen.Nature communications · 2026Article
- Leptospirosis-associated pulmonary hemorrhagic syndrome: immune mechanisms, clinical manifestations, and experimental models.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Review
- Development of leptospiral virulence-modifying protein detection assay: implications for pathogenesis and diagnostic test development.Microbiology spectrum · 2025Article
- Distinct strategies of epithelial cell barrier disruption by Leptospira interrogans isolated from human patients in Okinawa, Japan.PLoS neglected tropical diseases · 2025Article
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
- Application of Genomic Selection in Beef Cattle Disease Prevention.Animals : an open access journal from MDPI · 2025Review
- A Tale of Two Islands - Leptospirosis in Strikingly Different Ecological Settings Reveals Pathogenomic Insights.The American journal of tropical medicine and hygiene · 2024Article
- Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion.The protein journal · 2023Article
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.BMC infectious diseases · 2023Article
- Comparison of the PF07598-Encoded Virulence-Modifying Proteins ofTropical medicine and infectious disease · 2022Review
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- PathogenicFrontiers in microbiology · 2022Article
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- Characterization of a virulence-modifying protein ofFrontiers in microbiology · 2022Article
- Vaccination WithFrontiers in cellular and infection microbiology · 2022Article
- Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate.Human vaccines & immunotherapeutics · 2020Article
- Zeptomole per milliliter detection and quantification of edema factor in plasma by LC-MS/MS yields insights into toxemia and the progression of inhalation anthrax.Analytical and bioanalytical chemistry · 2019Article
- Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Leptospirosis is a globally important, neglected zoonotic infection caused by spirochetes of the genus Leptospira. Since genetic transformation remains technically limited for pathogenic Leptospira, a systems biology pathogenomic approach was used to infer leptospiral virulence genes by whole genome comparison of culture-attenuated Leptospira interrogans serovar Lai with its virulent, isogenic parent. Among the 11 pathogen-specific protein-coding genes in which non-synonymous mutations were found, a putative soluble adenylate cyclase with host cell cAMP-elevating activity, and two members of a previously unstudied ∼15 member paralogous gene family of unknown function were identified. This gene family was also uniquely found in the alpha-proteobacteria Bartonella bacilliformis and Bartonella australis that are geographically restricted to the Andes and Australia, respectively. How the pathogenic Leptospira and these two Bartonella species came to share this expanded gene family remains an evolutionary mystery. In vivo expression analyses demonstrated up-regulation of 10/11 Leptospira genes identified in the attenuation screen, and profound in vivo, tissue-specific up-regulation by members of the paralogous gene family, suggesting a direct role in virulence and host-pathogen interactions. The pathogenomic experimental design here is generalizable as a functional systems biology approach to studying bacterial pathogenesis and virulence and should encourage similar experimental studies of other pathogens.
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