ArticlePLoS neglected tropical diseases2013
Proteomic analysis of the excretory-secretory products from larval stages of Ascaris suum reveals high abundance of glycosyl hydrolases.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 83 citations in OpenAlex.
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- Among-population proteomic differences in Schistocephalus solidus based on excretory/secretory and total body protein predictions.Parasites & vectors · 2025Article
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- Quantifying metabolic activity of Ascaris suum L3 using resazurin reduction.Parasites & vectors · 2023Article
- A comparative 'omics' approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens.PLoS neglected tropical diseases · 2023Article
- Functional Diversity of the Excretory/Secretory Proteins of Nematode Parasites.Acta parasitologica · 2022Review
- Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae ofInternational journal of molecular sciences · 2022Article
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- Mining nematode protein secretomes to explain lifestyle and host specificity.PLoS neglected tropical diseases · 2021Article
- Characterization of the β-tubulin gene family in Ascaris lumbricoides and Ascaris suum and its implication for the molecular detection of benzimidazole resistance.PLoS neglected tropical diseases · 2021Article
- Lectin-Mediated Bacterial Modulation by the Intestinal NematodeInternational journal of molecular sciences · 2021Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAscaris lumbricoides and Ascaris suum are socioeconomically important and widespread parasites of humans and pigs, respectively. The excretory-secretory (ES) molecules produced and presented at the parasite-host interface during the different phases of tissue invasion and migration are likely to play critical roles in the induction and development of protective immune and other host responses. METHODOLOGY/PRINCIPAL
findingsThe aim of this study was to identify the ES proteins of the different larval stages (L3-egg, L3-lung and L4) by LC-MS/MS. In total, 106 different proteins were identified, 20 in L3-egg, 45 in L3-lung stage and 58 in L4. Although most of the proteins identified were stage-specific, 15 were identified in the ES products of at least two stages. Two proteins, i.e. a 14-3-3-like protein and a serpin-like protein, were present in the ES products from the three different larval stages investigated. Interestingly, a comparison of ES products from L4 with those of L3-egg and L3-lung showed an abundance of metabolic enzymes, particularly glycosyl hydrolases. Further study indicated that most of these glycolytic enzymes were transcriptionally upregulated from L4 onwards, with a peak in the adult stage, particularly in intestinal tissue. This was also confirmed by enzymatic assays, showing the highest glycosidase activity in protein extracts from adult worms gut. CONCLUSIONS/SIGNIFICANCE: The present proteomic analysis provides important information on the host-parasite interaction and the biology of the migratory stages of A. suum. In particular, the high transcriptional upregulation of glycosyl hydrolases from the L4 stage onwards reveals that the degradation of complex carbohydrates forms an essential part of the energy metabolism of this parasite once it establishes in the small intestine.
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