ArticlePloS one2013
Phanta: a non-fluorescent photochromic acceptor for pcFRET.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Enhancing the erythritol production of Yarrowia lipolytica by high-throughput screening based on highly sensitive artificial sensor and anchor protein cwp2.Journal of industrial microbiology & biotechnology · 2024Article
- Article
- ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells.Scientific reports · 2019Article
- ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.Scientific reports · 2017Article
- Dual observation of the ATP-evoked small GTPase activation and CaScientific reports · 2016Article
- A Guide to Fluorescent Protein FRET Pairs.Sensors (Basel, Switzerland) · 2016Review
- A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer.Scientific reports · 2015Article
- Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering.Proteins · 2015Article
- X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.PloS one · 2015Article
- Phototransformable fluorescent proteins: which one for which application?Histochemistry and cell biology · 2014Review
- Fluorescence lifetime imaging microscopy in the medical sciences.Protoplasma · 2014Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.
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Registered trials
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