ArticlePloS one2013
Monooxygenase, a novel beta-cypermethrin degrading enzyme from Streptomyces sp.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Comparative Genomics and Phylogenomics of Novel Radiation-Resistant BacteriumMicroorganisms · 2025Article
- Adsorption of Pyrethroids in Water by Calcined Shell Powder: Preparation, Characterization, and Mechanistic Analysis.Materials (Basel, Switzerland) · 2023Article
- Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9.AMB Express · 2022Article
- Analysis of Biodegradation of the Synthetic Pyrethroid Cypermethrin by Beauveria bassiana.Current microbiology · 2022Article
- Identification of FadT as a Novel Quorum Quenching Enzyme for the Degradation of Diffusible Signal Factor inInternational journal of molecular sciences · 2021Article
- Biodegradation of Allethrin by a Novel FungusMicroorganisms · 2020Article
- Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway inMicroorganisms · 2020Article
- Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides.Frontiers in microbiology · 2019Review
- Cyhalothrin biodegradation in Cunninghamella elegans.Environmental science and pollution research international · 2019Article
- Identification and immobilization of a novel cold-adapted esterase, and its potential for bioremediation of pyrethroid-contaminated vegetables.Microbial cell factories · 2017Article
- Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability.Microbial cell factories · 2017Article
- Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance.International journal of molecular sciences · 2016Article
- Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widely used insecticide beta-cypermethrin has become a public concern because of its environmental contamination and toxic effects on mammals. In this study, a novel beta-cypermethrin degrading enzyme designated as CMO was purified to apparent homogeneity from a Streptomyces sp. isolate capable of utilizing beta-cypermethrin as a growth substrate. The native enzyme showed a monomeric structure with a molecular mass of 41 kDa and pI of 5.4. The enzyme exhibited the maximal activity at pH 7.5 and 30°C. It was fairly stable in the pH range from 6.5-8.5 and at temperatures below 10°C. The enzyme activity was significantly stimulated by Fe(2+), but strongly inhibited by Ag(+), Al(3+), and Cu(2+). The enzyme catalyzed the degradation of beta-cypermethrin to form five products via hydroxylation and diaryl cleavage. A novel beta-cypermethrin detoxification pathway was proposed based on analysis of these products. The purified enzyme was identified as a monooxygenase by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry analysis (MALDI-TOF-MS) and N-terminal protein sequencing. Given that all the characterized pyrethroid-degrading enzymes are the members of hydrolase family, CMO represents the first pyrethroid-degrading monooxygenase identified from environmental microorganisms. Taken together, our findings depict a novel pyrethroid degradation mechanism and indicate that the purified enzyme may be a promising candidate for detoxification of beta-cypermethrin and environmental protection.
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