ArticlePloS one2013
Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Cryptic sex in Leishmania depends on SPO11 paralogs.PLoS pathogens · 2026Article
- Cryptic Sex inbioRxiv : the preprint server for biology · 2026Article
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Amoebiasis: Advances in Diagnosis, Treatment, Immunology Features and the Interaction with the Intestinal Ecosystem.International journal of molecular sciences · 2023Review
- Episomal and chromosomal DNA replication and recombination inFrontiers in molecular biosciences · 2023Review
- A Novel Spo11 Homologue Functions as a Positive Regulator in Cyst Differentiation inInternational journal of molecular sciences · 2021Article
- Detection and Sequencing of Iron Superoxide Dismutase Gene inArchives of Razi Institute · 2021Article
- Development of a CRISPR/Cas9 system in Entamoeba histolytica: proof of concept.International journal for parasitology · 2021Article
- Article
- Transcriptomic analysis reveals novel downstream regulatory motifs and highly transcribed virulence factor genes of Entamoeba histolytica.BMC genomics · 2019Article
- Advances inFrontiers in microbiology · 2019Review
- AIG1 affects in vitro and in vivo virulence in clinical isolates of Entamoeba histolytica.PLoS pathogens · 2018Article
- Cellular Events of Multinucleated Giant Cells Formation During the Encystation ofFrontiers in cellular and infection microbiology · 2018Article
- Genetic Evidence for Sexuality in Cochliopodium (Amoebozoa).The Journal of heredity · 2017Article
- Amoebozoans Are Secretly but Ancestrally Sexual: Evidence for Sex Genes and Potential Novel Crossover Pathways in Diverse Groups of Amoebae.Genome biology and evolution · 2017Article
- Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors.Frontiers in microbiology · 2017Review
- Deubiquitinases and cancer: A snapshot.Critical reviews in oncology/hematology · 2016Review
- Sexual reproduction and genetic exchange in parasitic protists.Parasitology · 2015Review
- Entamoeba histolytica Dmc1 Catalyzes Homologous DNA Pairing and Strand Exchange That Is Stimulated by Calcium and Hop2-Mnd1.PloS one · 2015Article
- Evolutionary genomics and population structure of Entamoeba histolytica.Computational and structural biotechnology journal · 2014Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. Here we show the up regulation of mitotic and meiotic HR genes in Entamoeba during serum starvation, and encystation. To directly demonstrate HR we use a simple PCR-based method involving inverted repeats, which gives a reliable read out, as the recombination junctions can be determined by sequencing the amplicons. Using this read out, we demonstrate enhanced HR under growth stress in E. histolytica, and during encystation in E. invadens. We also demonstrate recombination between chromosomal inverted repeats. This is the first experimental demonstration of HR in Entamoeba and will help future investigations into this process, and to explore the possibility of meiosis in Entamoeba.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.