ArticlePloS one2013
Characteristic and functional analysis of toll-like receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, reveals ancient origin of TLR-mediated innate immunity.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 116 citations in OpenAlex.
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- Evolution of the Major Components of Innate Immunity in Animals.Journal of molecular evolution · 2024Review
- The Modification of H3K4me3 Enhanced the Expression ofInternational journal of molecular sciences · 2024Article
- Pattern recognition receptors in Crustacea: immunological roles under environmental stress.Frontiers in immunology · 2024Review
- The characterization of toll-like receptor repertoire inEcology and evolution · 2023Article
- Investigation of Immune Responses in Giant African Snail,International journal of molecular sciences · 2023Article
- Bivalves Present the Largest and Most Diversified Repertoire of Toll-Like Receptors in the Animal Kingdom, Suggesting Broad-Spectrum Pathogen Recognition in Marine Waters.Molecular biology and evolution · 2023Article
- Antiviral effects of interferon-stimulated genes in bats.Frontiers in cellular and infection microbiology · 2023Review
- Hippo dictates signaling for cellular homeostasis and immune defense inFrontiers in immunology · 2023Article
- Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review.Frontiers in immunology · 2023Review
- In search of the Aplysia immunome: an in silico study.BMC genomics · 2022Article
- Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oysterFrontiers in immunology · 2022Article
- The evolution of the metazoan Toll receptor family and its expression during protostome development.BMC ecology and evolution · 2021Article
- Article
- Review
- Toll-Like Receptors: General Molecular and Structural Biology.Journal of immunology research · 2021Review
- Virulence ofFrontiers in immunology · 2021Article
- NF-κB signaling induces inductive expression of the downstream molecules and IgD gene in the freshwater carp,3 Biotech · 2020Article
- Participation of Endosomes in Toll-Like Receptor 3 Transportation Pathway in Murine Astrocytes.Frontiers in cellular neuroscience · 2020Article
- Fundamental Characteristics of Bat Interferon Systems.Frontiers in cellular and infection microbiology · 2020Review
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolution of TLR-mediated innate immunity is a fundamental question in immunology. Here, we report the characterization and functional analysis of four TLR members in the lophotrochozoans Crassostreagigas (CgTLRs). All CgTLRs bear a conserved domain organization and have a close relationship with TLRs in ancient non-vertebrate chordates. In HEK293 cells, every CgTLR could constitutively activate NF-κB responsive reporter, but none of the PAMPs tested could stimulate CgTLR-activated NF-κB induction. Subcellular localization showed that CgTLR members have similar and dual distribution on late endosomes and plasma membranes. Moreover, CgTLRs and CgMyD88 mRNA show a consistent response to multiple PAMP challenges in oyster hemocytes. As CgTLR-mediated NF-κB activation is dependent on CgMyD88, we designed a blocking peptide for CgTLR signaling that would inhibit CgTLR-CgMyD88 dependent NF-κB activation. This was used to demonstrate that a Vibrio parahaemolyticus infection-induced enhancement of degranulation and increase of cytokines TNF mRNA in hemocytes, could be inhibited by blocking CgTLR signaling. In summary, our study characterized the primitive TLRs in the lophotrocozoan C. gigas and demonstrated a fundamental role of TLR signaling in infection-induced hemocyte activation. This provides further evidence for an ancient origin of TLR-mediated innate immunity.
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